Detection of Merkel Cell Polyomavirus in the Human Tissues from 41 Japanese Autopsy Cases Using Polymerase Chain Reaction
Detection of Merkel Cell Polyomavirus in the Human Tissues from 41 Japanese Autopsy Cases Using Polymerase Chain Reaction
复制标题
DOI：
10.1159/000338620
复制
发表时间：
2013-01-01
期刊：
影响因子：
4.6
通讯作者：
Hayashi, Kazuhiko
中科院分区：
文献类型：
--
作者：
Matsushita, Michiko;Kuwamoto, Satoshi;Hayashi, Kazuhiko
最近显示，约80%的默克尔细胞癌携带一种名为默克尔细胞多瘤病毒（MCPyV）的新型多瘤病毒。已在人体组织样本中检测到MCPyV。然而，MCPyV在非肿瘤性日本人体组织中的详细分布仍不清楚。为了解决这一问题，我们使用了单一或实时定量聚合酶链反应（PCR）的41例尸检病例。PCR显示非肿瘤样本中的MCPyV-DNA：总计，29/41（71%）;成人，29/39（74%）;胎儿或婴儿，0/2;男性，24/28（86%）;女性，5/13（38%）;总人体组织，66/572（12%）;皮肤，8/15（53%）;肾上腺9/33（27%），其它16个器官（4-25%）。本研究首次报道了MCPyV-DNA在甲状腺、肾上腺、脾、骨髓、胃、胆囊、胰腺、心脏和主动脉的非肿瘤组织中的存在。PCR显示，与默克尔细胞癌样品相比，所有MCPyV阳性组织中的病毒载量范围为0.00026至0.22。这些详细的PCR数据显示，日本男性中MCPyV感染的患病率高于女性（p = 0.004），并且与先前的报告相比，MCPyV在更多非肿瘤性人体组织中广泛分布，病毒载量较低。这些数据为进一步研究MCPyV感染和MCPyV相关疾病提供了有价值的见解。版权所有（c）2012 S. Karger AG，巴塞尔
It has recently been shown that approximately 80% of Merkel cell carcinomas harbor a novel polyomavirus named Merkel cell polyomavirus (MCPyV). MCPyV has been detected in human tissue samples. However, detailed distribution of MCPyV in non-neoplastic Japanese human tissues remains unclear. To address this, we used single or real-time quantitative polymerase chain reaction (PCR) for 41 autopsy cases. PCR revealed MCPyV-DNA in non-neoplastic samples: total, 29/41 (71%); adult, 29/39 (74%); fetus or infant, 0/2; men, 24/28 (86%); women, 5/13 (38%); total human tissues, 66/572 (12%); skin, 8/15 (53%); adrenal gland, 9/33 (27%), and other 16 organs (4-25%). This study first reported the presence of MCPyV-DNA in non-neoplastic tissues of thyroid gland, adrenal gland, spleen, bone marrow, stomach, gallbladder, pancreas, heart, and aorta. PCR revealed that viral load ranged from 0.00026 to 0.22 in all MCPyV-positive tissues compared with Merkel cell carcinoma samples. These detailed PCR data showed higher prevalence of MCPyV infection in Japanese men than women ( p = 0.004) and broad distribution of MCPyV with low viral load in more non-neoplastic human tissues than in the previous reports. These data provide valuable insights for further studies of MCPyV infection and MCPyV-related diseases. Copyright (c) 2012 S. Karger AG, Basel