Effects of central irisin administration on the uncoupling proteins in rat brain
Effects of central irisin administration on the uncoupling proteins in rat brain
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DOI：
10.1016/j.neulet.2016.02.046
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发表时间：
2016-04-08
影响因子：
2.5
通讯作者：
Kirbag, Sevda
中科院分区：
文献类型：
--
作者：
Erden, Yavuz;Tekin, Suat;Kirbag, Sevda
鸢尾素是一种产热肽，通过激活UCP 1使白色脂肪组织发育为棕色脂肪组织。本研究旨在确定鸢尾素对UCP的影响。研究中使用Sprague道利雌性大鼠。向大鼠脑室内注射1、3和10 μ M浓度的鸢尾素，对照组仅接受赋形剂。在16、24和48 h时间间隔处死动物，取出其脑。分离下丘脑、垂体、海马、小脑、纹状体和皮质区，测定UCP 2、UCP 3、UCP 4和UCP 5 mRNA水平。结果表明，高剂量鸢尾素处理后，各脑区UCP 5 mRNA表达均升高（p < 0.05），三个剂量组UCP 4表达均降低（除垂体外，p < 0.05）。UCP 2和UCP 3 mRNA在小脑和纹状体的表达显著增加（p < 0.05）。下丘脑、垂体、海马和皮质区UCP 2 mRNA表达降低（p < 0.05）。还观察到大鼠体温随注射鸢尾素而升高，并且这种升高在24小时最显著（p < 0.05）。本研究结果提示，UCP 2 -5在脑的不同区域均有表达，而鸢尾素影响UCP 2 - 5的表达，可能在脑的某些功能中发挥有效作用。(C)2016爱思唯尔爱尔兰有限公司版权所有。
Irisin is a thermogenic peptide that enables the development of brown adipose tissue from white adipose tissue by activating the UCP1. This study has been designed to determine the effects of the irisin on UCPs. Sprague Dawley female rats were used in the study. 1, 3 and 10 mu M concentrations of irisin were injected intracerebroventricularly to the rats, and the control group was received only vehicle. The animals were killed at the 16, 24, and 48 h time intervals and their brains were taken out. The hypothalamus, pituitary gland, hippocampus, cerebellum, striatum and cortex areas were separated and the UCP2, UCP3, UCP4 and UCP5 mRNA levels were determined. Just before the animals were killed, their body temperatures were recorded.It was observed that after application of the high dose irisin, UCP5 mRNA level in the all brain areas increased (p < 0.05); it was also observed that the three doses decreased the UCP4 expression in all brain areas (except the pituitary gland; p < 0.05). The UCP2 and UCP3 mRNA expressions showed significantly increase in cerebellum and striatum (p < 0.05). The UCP2 mRNA expression decreased in hypothalamus, pituitary gland, hippocampus and cortex areas (p < 0.05). It was also observed that the body temperatures of the rats increased depending on the irisin injection and this increase was the most considerable at the 24h (p < 0.05). The results of this study suggest that the UCP2-5 is expressed in different areas of the brain, and the irisin affects this expression, and may have effective roles in some brain functions. (C) 2016 Elsevier Ireland Ltd. All rights reserved.