Flow dichroism evidence for tilting of the bases when DNA is in solution.
Flow dichroism evidence for tilting of the bases when DNA is in solution.
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DNA 处于溶液中时碱基倾斜的流动二色性证据。
DOI：
10.1073/pnas.80.8.2193
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发表时间：
1983
影响因子：
11.1
通讯作者：
JohnsonJr,WC
中科院分区：
文献类型：
--
作者：
Dougherty,AM;Causley,GC;JohnsonJr,WC
从产气荚膜梭菌、大肠杆菌和黄色微球菌的DNA的流动线性二色性研究扩展到真空紫外区到175 nm。与研究较好的长波长pi区相比，短波长pi区表现出较少的负约化线性二色性。这些结果在0.001~1.0M范围内与DNA的类型和盐的浓度无关，这证明碱基不垂直于流动方向。假设DNA倾向于沿着螺旋轴排列，碱基可以与螺旋轴形成的最大角度是73+/-3度。
Flow linear dichroism studies of DNAs from Clostridium perfringens, Escherichia coli, and Micrococcus luteus are extended into the vacuum UV region to 175 nm. The short-wavelength pi pi region shows a less negative reduced linear dichroism than the well-studied long-wavelength pi pi region. These results are independent of the type of DNA and the concentration of salt over the range 0.001 to 1.0 M. This proves that the bases are not perpendicular to the flow orientation. Assuming that the DNA tends to align along its helix axis, the largest angle that the bases can make with the helix axis is 73 +/- 3 degrees.