X-ray radiation-induced damage in DNA monitored by online Raman
X-ray radiation-induced damage in DNA monitored by online Raman
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DOI：
10.1107/s0909049506043251
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发表时间：
2007-01-01
影响因子：
2.5
通讯作者：
Ravelli, Raimond B. G.
中科院分区：
文献类型：
--
作者：
McGeehan, John E.;Carpentier, Philippe;Ravelli, Raimond B. G.
在大分子晶体学（MX）数据收集之前、期间和之后，在冷氮气流中保存100 K的单晶DNA上收集了高质量的拉曼光谱。8-溴-2'-脱氧鸟苷中C-Br键的非对称弯曲振动在293 cm（-1）的非共振拉曼光谱中被明确地识别出来。在x射线照射过程中，可以通过连续记录拉曼光谱来监测该键的断裂，并且其随剂量的衰减可以直接与MX确定的电子密度图中溴化物定义的损失相关。
High-quality Raman spectra have been collected on single DNA crystals kept at 100 K in a flow of cold nitrogen gas prior, during and after macromolecular crystallography (MX) data collection. The non-symmetric bending vibration for C-Br bonds in 8-bromo-2'-deoxyguanosine has been unambiguously identified at 293 cm(-1) in the non-resonant Raman spectra. The breakage of this bond could be monitored by the continuous recording of Raman spectra during X-ray exposure, and its decay versus dose could be directly correlated with the loss of the bromide definition within the electron-density maps as determined with MX.