Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields
Spatial patterning of endothelial cells and vascular network formation using ultrasound standing wave fields
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DOI：
10.1121/1.4812867
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发表时间：
2013-08-01
影响因子：
2.4
通讯作者：
Hocking, Denise C.
中科院分区：
文献类型：
--
作者：
Garvin, Kelley A.;Dalecki, Diane;Hocking, Denise C.
细胞的空间组织对于正确的组织组装和器官功能至关重要。因此，复杂组织和器官的成功工程需要在三个维度上控制细胞组织的方法。特别是，在三维组织结构中促进内皮细胞排列和血管网络形成的技术将提供一种为新形成的组织提供必需的氧气和营养的方法。与超声驻波场相关的声辐射力可以快速且非侵入性地将细胞组织成三维胶原凝胶内的不同的多细胞平面带。本文呈现的结果表明，三维胶原凝胶内内皮细胞的空间模式可以通过声场的声学参数的设计来控制。使用不同的超声驻波场暴露参数将内皮细胞组织成松散聚集或密集的平面带。血管形成的速率和所得内皮细胞网络的形态受到超声诱导的细胞平面带的初始密度的影响。超声驻波场提供了一种快速、非侵入性的方法来在三维上形成细胞图案，并直接在工程组织结构内形成血管网络和形态。 (C) 2013 年美国声学学会。
The spatial organization of cells is essential for proper tissue assembly and organ function. Thus, successful engineering of complex tissues and organs requires methods to control cell organization in three dimensions. In particular, technologies that facilitate endothelial cell alignment and vascular network formation in three-dimensional tissue constructs would provide a means to supply essential oxygen and nutrients to newly forming tissue. Acoustic radiation forces associated with ultrasound standing wave fields can rapidly and non-invasively organize cells into distinct multicellular planar bands within three-dimensional collagen gels. Results presented herein demonstrate that the spatial pattern of endothelial cells within three-dimensional collagen gels can be controlled by design of acoustic parameters of the sound field. Different ultrasound standing wave field exposure parameters were used to organize endothelial cells into either loosely aggregated or densely packed planar bands. The rate of vessel formation and the morphology of the resulting endothelial cell networks were affected by the initial density of the ultrasound-induced planar bands of cells. Ultrasound standing wave fields provide a rapid, non-invasive approach to pattern cells in three-dimensions and direct vascular network formation and morphology within engineered tissue constructs. (C) 2013 Acoustical Society of America.