ExoTracker: a low-pH-activatable fluorescent probe for labeling exosomes and monitoring endocytosis and trafficking
ExoTracker: a low-pH-activatable fluorescent probe for labeling exosomes and monitoring endocytosis and trafficking
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DOI：
10.1039/d0cc06208a
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发表时间：
2020-12-07
影响因子：
4.9
通讯作者：
Guan, Feng
中科院分区：
文献类型：
--
作者：
Zhou, Xiaoman;Zhang, Jianjian;Guan, Feng
外泌体（Exosomes）是一种大小在30-100 nm之间的纳米级细胞外囊泡，通过传递功能性生物分子介导细胞间的信息传递，在肿瘤的发生、发展等多种生理、病理过程中发挥重要作用。非常需要用于在细胞-细胞通信中可视化和跟踪外泌体的更多新的和有效的技术。然而，常用的外来体标记探针的应用受到特异性和严格的pH耐受性的限制。我们在这里描述了一种新的外泌体标记荧光探针的构建和测试，称为“ExoTracker”，它在酸性环境中显示出低细胞毒性和高荧光强度。ExoTracker用于在细胞内吞作用中有效跟踪外泌体。
Exosomes (a type of nanoscale extracellular vesicle with a size range of 30-100 nm) mediate cell-cell communication by transferring functional biomolecules, and play an important role in various physiological and pathological processes, including tumor development and progression. More new and effective techniques for visualizing and tracking exosomes in cell-cell communication are highly desirable. However, the application of commonly used exosome-labeling probes is limited by the need for specificity and strict pH tolerance. We describe here the construction and testing of a novel exosome labeling fluorescent probe termed as "ExoTracker", which displayed low cytotoxicity and a high fluorescence intensity in acidic environments. ExoTracker was applied for effective tracking of exosomes in cell endocytosis.