Granulocyte-macrophage colony-stimulating factor enhances wound healing in diabetes via upregulation of proinflammatory cytokines
Granulocyte-macrophage colony-stimulating factor enhances wound healing in diabetes via upregulation of proinflammatory cytokines
复制标题
粒细胞巨噬细胞集落刺激因子通过上调促炎细胞因子促进糖尿病伤口愈合
DOI：
10.1111/j.1365-2133.2009.09528.x
复制
发表时间：
2010-03-01
影响因子：
10.3
通讯作者：
Bao, S.
中科院分区：
文献类型：
--
作者：
Fang, Y.;Shen, J.;Bao, S.
背景慢性溃疡，尤其是糖尿病患者，仍然是一个重要的临床问题。外源性粒细胞-巨噬细胞集落刺激因子（GM-CSF）对慢性创面愈合有明显的治疗作用，但其在糖尿病创面愈合中的作用尚待进一步研究。目的利用糖尿病小鼠模型，探讨GM-CSF在创面愈合中的作用。结果与非糖尿病患者相比，糖尿病患者伤口中GM-CSF的产生显著减少（50%）。外源性GM-CSF显著促进了糖尿病小鼠的伤口愈合，并伴随着白细胞介素-6和单核细胞趋化蛋白-1的产生增加。升高的细胞因子与增加的新生血管形成、巨噬细胞和中性粒细胞浸润相关。GM-CSF在非糖尿病伤口愈合中没有表现出有益的作用。结论我们的研究结果为糖尿病慢性溃疡的临床治疗提供了有用的指导。
Background Chronic ulceration, especially in diabetes, remains a substantial clinical problem. Exogenous granulocyte-macrophage colony-stimulating factor (GMCSF) is efficacious in the treatment of chronic wound healing in both animal models and patients, but its role in diabetic wounds remains to be explored.Objectives Using a diabetic mouse model, to investigate the role of GM-CSF in wound healing.Methods Clinical observation, histopathology, immunohistochemistry and cytokine assays.Results There was a significant reduction (50%) in GM-CSF production in the wounds of the diabetics compared with nondiabetics. Exogenous GM-CSF substantially enhanced the wound healing in diabetic mice, accompanied by increased interleukin-6 and monocyte chemoattractant protein-1 production. The elevated cytokines correlated with increased neovascularization, and infiltration of macrophages and neutrophils. GM-CSF showed no beneficial effects in nondiabetic wound healing.Conclusions Our results provide useful guidelines for the clinical management of chronic ulceration in diabetes.