Role of a Streptococcus gordonii copper-transport operon, copYAZ, in biofilm detachment
Role of a Streptococcus gordonii copper-transport operon, copYAZ, in biofilm detachment
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DOI：
10.1111/j.1399-302x.2004.00176.x
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发表时间：
2004-12-01
影响因子：
--
通讯作者：
Ganeshkumar, N
中科院分区：
文献类型：
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作者：
Mitrakul, K;Loo, CY;Ganeshkumar, N
戈登链球菌是一种与牙菌斑形成有关的口腔细菌。位于S. gordonii的copA操纵子是三个开放的阅读框架，分别命名为copY、copA和copZ。这些开放阅读框与变形链球菌中涉及铜稳态和生物膜脱离的copYAZ基因同源。本研究探讨copYAZ基因是否在链球菌生物膜形成和脱落中发挥作用。gordonii。copY基因编码一个143个氨基酸的蛋白，与铜转运操纵子的负转录调节因子同源，copA基因编码一个748个氨基酸的铜转运P型ATP酶，copZ基因编码一个69个氨基酸的推定金属伴侣蛋白。变异人在S.通过插入突变使Gordonii失活，并检查每种突变体的生长、生物膜形成和脱离。S.戈登氏菌copY、copA和copZ突变体能够在聚苯乙烯和玻璃表面上形成生物膜。然而，copZ的失活和较小程度的copY导致生物膜脱离缺陷的表型，这与先前在S.表明微量元素铜可能影响细菌生物膜的生物膜剥离。
Streptococcus gordonii is a pioneer oral bacterium that is associated with the initiation of dental plaque development. Located downstream of the S. gordonii adc operon, which is involved in competence and biofilm formation, were three open reading frames, designated copY, copA and copZ. These open reading frames were homologous to the copYAZ genes in Streptococcus mutans that are involved in copper homeostasis and biofilm detachment. This study examined whether copYAZ genes play any role in the biofilm formation and detachment of S. gordonii. The copY gene encodes a 143-amino acid protein homologous to the negative transcriptional regulator of a copper-transport operon, copA encodes a 748-amino acid copper-transporting P-type ATPase, and copZ encodes a 69-amino acid putative metallochaperone protein in S. mutans. Each open reading frame in the copYAZ operon in S. gordonii was inactivated by insertional mutation and the growth, biofilm formation and detachment of each mutant were examined. S. gordonii copY::spec(R), copA::spec(R), and copZ::spec(R) mutants were able to form biofilms on both polystyrene and glass surfaces. However, inactivation of copZ and to a lesser extent copY resulted in phenotypes that were defective in biofilm detachment, which is consistent with previous observations in S. mutans and suggests that the trace element copper might influence biofilm detachment of bacterial biofilms.