GABAergic and glycinergic inhibition modulate monaural auditory response properties in the avian superior olivary nucleus
GABAergic and glycinergic inhibition modulate monaural auditory response properties in the avian superior olivary nucleus
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DOI：
10.1152/jn.01088.2010
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发表时间：
2011-05-01
影响因子：
2.5
通讯作者：
Burger, R. M.
中科院分区：
文献类型：
--
作者：
Coleman, W. L.;Fischl, M. J.;Burger, R. M.
科尔曼WL，Fischl MJ，Weimann SR，Burger RM. GABA能和甘氨酸能抑制调节鸟类上级橄榄核的单耳听觉反应特性。J Neurophysiol 105：2405-2420，2011.首次发表于2011年3月2日; doi：10.1152/jn.01088.2010.-上级橄榄核（SON）是鸟类听觉脑干抑制的主要来源。虽然对SON靶核的抑制作用了解很多，但对SON本身如何处理听觉信息或抑制如何调节这些特性知之甚少。此外，尚未描述SON内抑制性输入的突触生理学。我们研究了这些问题，在体内和体外电生理技术结合免疫组织化学在鸡，一个有机体的听觉脑干，否则已被很好地表征。我们提供了一个彻底的表征单耳响应特性的SON和抑制性输入在塑造这些功能的影响。我们发现，SON包含一个异构的混合物的响应模式，以声刺激，在大多数神经元，这些反应调制的GABA能和甘氨酸抑制输入。有趣的是，许多SON神经元调谐到低频率具有强大的锁相能力，并且这种锁相的精度通过抑制性输入来增强。在突触水平上，我们发现SON内的诱发和自发抑制性突触后电流（IPSC）也由所有测试神经元中的GABA能和甘氨酸能抑制介导。自发IPSC的分析表明，大多数SON细胞接收纯GABA能终端的混合物，以及GABA和甘氨酸共同释放的终端。SON内甘氨酸能信号传导的证据是一个新的结果，对于理解听觉脑干中的抑制功能具有重要意义。
Coleman WL, Fischl MJ, Weimann SR, Burger RM. GABAergic and glycinergic inhibition modulate monaural auditory response properties in the avian superior olivary nucleus. J Neurophysiol 105: 2405-2420, 2011. First published March 2, 2011; doi:10.1152/jn.01088.2010.-The superior olivary nucleus (SON) is the primary source of inhibition in the avian auditory brainstem. While much is known about the role of inhibition at the SON's target nuclei, little is known about how the SON itself processes auditory information or how inhibition modulates these properties. Additionally, the synaptic physiology of inhibitory inputs within the SON has not been described. We investigated these questions using in vivo and in vitro electrophysiological techniques in combination with immunohistochemistry in the chicken, an organism for which the auditory brainstem has otherwise been well characterized. We provide a thorough characterization of monaural response properties in the SON and the influence of inhibitory input in shaping these features. We found that the SON contains a heterogeneous mixture of response patterns to acoustic stimulation and that in most neurons these responses are modulated by both GABAergic and glycinergic inhibitory inputs. Interestingly, many SON neurons tuned to low frequencies have robust phase-locking capability and the precision of this phase locking is enhanced by inhibitory inputs. On the synaptic level, we found that evoked and spontaneous inhibitory postsynaptic currents (IPSCs) within the SON are also mediated by both GABAergic and glycinergic inhibition in all neurons tested. Analysis of spontaneous IPSCs suggests that most SON cells receive a mixture of both purely GABAergic terminals, as well as terminals from which GABA and glycine are coreleased. Evidence for glycinergic signaling within the SON is a novel result that has important implications for understanding inhibitory function in the auditory brainstem.