2 ALTERNATIVE PATHWAYS OF DOUBLE-STRAND BREAK REPAIR THAT ARE KINETICALLY SEPARABLE AND INDEPENDENTLY MODULATED
2 ALTERNATIVE PATHWAYS OF DOUBLE-STRAND BREAK REPAIR THAT ARE KINETICALLY SEPARABLE AND INDEPENDENTLY MODULATED
复制标题
DOI：
10.1128/mcb.12.3.1292
复制
发表时间：
1992-03-01
影响因子：
5.3
通讯作者：
HABER, JE
中科院分区：
文献类型：
--
作者：
FISHMANLOBELL, J;RUDIN, N;HABER, JE
HO内切酶诱导的酿酒酵母双链断裂可以通过两种不同的竞争途径进行重组。 在含有直接重复序列的质粒中，其中一个重复序列被HO内切核酸酶切割位点中断，缺口修复产生基因转换，而单链退火产生缺失。 与单链退火机制的预测一致，缺失形成并不伴随着相互重组产物的形成。 当重复间隔的距离增加4.4kb时，缺失延迟60分钟。 此外，缺失形成的速率对应于互补区域变成单链的时间。 间隙修复过程是独立的距离，但减少在rad52突变体和G1期阻滞的细胞。
HO endonuclease-induced double-strand breaks in Saccharomyces cerevisiae can undergo recombination by two distinct and competing pathways. In plasmid containing a direct repeat, in which one repeat is interrupted by an HO endonuclease cut site, gap repair yields gene conversions while single-strand annealing produces deletions. Consistent with predictions of the single-strand annealing mechanism, deletion formation is not accompanied by the formation of reciprocal recombination product. Deletions are delayed 60 min when the distance separating the repeats is increased by 4.4 kb. Moreover, the rate of deletion formation corresponds to the time at which complementary regions become single stranded. Gap repair processes are independent of distance but are reduced in rad52 mutants and in G1-arrested cells.