Monoclonal antibodies to CD2 and lymphocyte function-associated antigen 3 inhibit human thymic epithelial cell-dependent mature thymocyte activation.
Monoclonal antibodies to CD2 and lymphocyte function-associated antigen 3 inhibit human thymic epithelial cell-dependent mature thymocyte activation.
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DOI：
10.4049/jimmunol.139.8.2573
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发表时间：
1987-10
影响因子：
4.4
通讯作者：
S. Denning;Debbi T. Tuck;L. Vollger;T. Springer;K. Singer;B. Haynes
中科院分区：
文献类型：
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作者：
S. Denning;Debbi T. Tuck;L. Vollger;T. Springer;K. Singer;B. Haynes
最近对人胸腺细胞-胸腺上皮（TE）细胞相互作用的研究表明，胸腺细胞与TE细胞结合，这种结合的结果是TE细胞为植物血凝素（PHA）诱导的成熟胸腺细胞活化提供辅助细胞信号。在本文中，我们报告的分子参与TE细胞依赖的成熟胸腺细胞活化的研究。PHA诱导的胸腺细胞活化所必需的TE-胸腺细胞相互作用被针对胸腺细胞上的分化簇（CD）2抗原和TE细胞上的淋巴细胞功能相关（LFA）-3抗原的单克隆抗体抑制。抗CD 2（α CD 2）和LFA-3（α LFA-3）抗原抗体对TE辅助细胞信号的抑制发生在胸腺细胞活化的早期，并阻止胸腺细胞白细胞介素2受体表达。此外，α CD 2和α LFA-3抑制PHA诱导的胸腺细胞活化在整个胸腺外植体培养物中，表明当PHA诱导的胸腺细胞触发的辅助细胞信号由完整胸腺微环境内的细胞递送时，CD 2和LFA-3抗原在胸腺细胞活化中的重要作用。
Recent study of human thymocyte-thymic epithelial (TE) cell interactions has demonstrated that thymocytes bind to TE cells, and a consequence of this binding is the provision of accessory cell signals by TE cells for phytohemagglutinin (PHA)-induced mature thymocyte activation. In this paper we report on studies of the molecules involved in TE cell-dependent mature thymocyte activation. TE-thymocyte interactions necessary for PHA-induced thymocyte activation were inhibited by monoclonal antibodies against the cluster of differentiation (CD)2 antigen on thymocytes and lymphocyte function-associated (LFA)-3 antigen on TE cells. Inhibition of TE accessory cell signals by antibodies against CD2 (alpha CD2) and LFA-3 (alpha LFA-3) antigens occurred early on during thymocyte activation and prevented thymocyte interleukin 2 receptor expression. Further, alpha CD2 and alpha LFA-3 inhibited PHA-induced thymocyte activation in whole thymic explant cultures suggesting a significant role of the CD2 and LFA-3 antigens in thymocyte activation when accessory cell signals for PHA-induced thymocyte triggering were delivered by cells within an intact thymic microenvironment.