Functional recovery in rat spinal cord injury induced by hyperbaric oxygen preconditioning
Functional recovery in rat spinal cord injury induced by hyperbaric oxygen preconditioning
复制标题
高压氧预处理致大鼠脊髓损伤的功能恢复
DOI：
10.1179/1743132812y.0000000096
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发表时间：
2012-12-01
影响因子：
1.9
通讯作者：
Feng, Hua
中科院分区：
文献类型：
--
作者：
Lu, Pei-Gang;Hu, Sheng-Li;Feng, Hua
背景：人们普遍认为，神经外科干预会导致手术本身不可避免的损伤，特别是对于髓内脊髓肿瘤。本研究旨在研究高压氧预处理（HBO-PC）是否对大鼠脊髓损伤（SCI）模型具有神经保护作用。方法：将sd - dawley大鼠随机分为3组：HBO-PC组、低压缺氧预处理（HH-PC）对照组和常压对照组。各组均采用减重装置进行脊髓损伤。观察各组大鼠的神经行为和电生理功能。采用免疫组织化学、tdt介导的dutp -生物素缺口末端标记和轴突束示踪等方法分析组织切片。结果：脊髓损伤后出现明显的神经功能缺损，HBO-PC可改善损伤后1周的神经功能缺损。脊髓损伤后运动诱发电位和躯体感觉诱发电位潜伏期明显延迟，HBO-PC和HH-PC可使其减弱。与常压对照组相比，高压氧预处理和低压缺氧预处理显著减少了tdt介导的dutp -生物素缺口末端标记阳性细胞数量，增加了巢蛋白阳性细胞数量。HBO-PC和HH-PC促进脊髓损伤后轴突生长。结论：高压氧和低氧预处理可促进脊髓损伤后的功能恢复和抑制细胞凋亡，可能是一种有效的神经外科脊髓损伤预防策略。
Background: It is a common belief that neurosurgical interventions can cause inevitable damage resulting from the procedure itself in surgery especially for intramedullary spinal cord tumors. The present study was designed to examine if hyperbaric oxygen preconditioning (HBO-PC) was neuroprotective against surgical injuries using a rat model of spinal cord injury (SCI).Methods: Sprague-Dawley rats were randomly divided into three groups: HBO-PC group, hypobaric hypoxic preconditioning (HH-PC) control group, and normobaric control group. All groups were subjected to SCI by weight drop device. Rats from each group were examined for neurological behavior and electrophysiological function. Tissue sections were analyzed by using immunohistochemistry, TdT-mediated dUTP-biotin nick end labeling, and axonal tract tracing.Results: Significant neurological deficits were observed after SCI and HBO-PC and HH-PC improved neurological deficits 1 week post-injury. The latencies of motor-evoked potential and somatosensory-evoked potential were significantly delayed after SCI, which was attenuated by HBO-PC and HH-PC. Compared with normobaric control group, pretreatment with HBO and hypobaric hypoxia significantly reduced the number of TdT-mediated dUTP-biotin nick end labeling-positive cells, and increased nestin-positive cells. HBO-PC and HH-PC enhanced axonal growth after SCI.Conclusions: In conclusion, preconditioning with HBO and hypobaric hypoxia can facilitate functional recovery and suppress cell apoptosis after SCI and may prove to be a useful preventive strategy to neurosurgical SCI.