Morin Inhibits Sortase A and Subsequent Biofilm Formation in Streptococcus mutans
Morin Inhibits Sortase A and Subsequent Biofilm Formation in Streptococcus mutans
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DOI：
10.1007/s00284-013-0439-x
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发表时间：
2014-01-01
影响因子：
2.6
通讯作者：
Chen, Wei Min
中科院分区：
文献类型：
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作者：
Huang, Ping;Hu, Ping;Chen, Wei Min
变形链球菌（S.变形杆菌）是龋齿的主要病原体，并通过分选酶A（SrtA）介导的细胞壁锚定蛋白Pac粘附于牙齿表面。SrtA活性的抑制导致S.变形菌和龋齿的频率。桑色素是一种天然植物提取物，以前曾报道过抑制金黄色葡萄球菌SrtA活性。在这里，我们证明了桑色素对S。变形杆菌UA 159 SrtA，IC 50为27.2 +/- A 2.6 μ M。Western blotting表明，30 μ M桑色素诱导部分释放的Pac蛋白到上清液中。S.在30 μ M桑色素存在下，变形杆菌的数量减少，这不是由S.变异体生存能力。这些结果表明桑色素作为一种新的防龋剂可能是重要的。
Streptococcus mutans (S. mutans) is the main etiological agent of dental caries, and adheres to the tooth surface through the sortase A (SrtA)-mediated cell wall-anchored protein Pac. Inhibition of SrtA activity results in a marked reduction in the adhesion potential of S. mutans, and the frequency of dental caries. Morin is a natural plant extract that was previously reported to inhibit Staphylococcus aureus SrtA activity. Here, we demonstrate that morin has an inhibitory effect against S. mutans UA159 SrtA, with an IC50 of 27.2 +/- A 2.6 mu M. Western blotting demonstrated that 30 mu M morin induced the partial release of the Pac protein into the supernatant. The biofilm mass of S. mutans was reduced in the presence of 30 mu M morin, which was not caused by a decrease in S. mutans viability. These results indicate that morin might be important as a new agent to prevent caries.