Sulfated glucuronic acid-containing glycoconjugates are temporally and spatially regulated antigens in the developing mammalian nervous system.
Sulfated glucuronic acid-containing glycoconjugates are temporally and spatially regulated antigens in the developing mammalian nervous system.
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含硫酸化葡萄糖醛酸的糖复合物是发育中的哺乳动物神经系统中的时间和空间调节抗原。
DOI：
10.1016/0012-1606(87)90104-7
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发表时间：
1987
影响因子：
2.7
通讯作者：
Yamamoto,M
中科院分区：
文献类型：
--
作者：
Schwarting,GA;Jungalwala,FB;Chou,DK;Boyer,AM;Yamamoto,M
单克隆抗体4F4，这是对胚胎大鼠前脑细胞悬浮液提出，反应与酸性糖脂和几个高分子量的糖蛋白在啮齿动物的大脑。主要的反应性糖脂在胚胎第15天（E15）表达最多，在大鼠出生后第14天（P14）不再检测到。4F4抗体与从人坐骨神经中分离的含葡萄糖醛酸和硫酸盐的脂质以及小鼠和大鼠胚胎脑组织中的脂质反应。虽然出生后糖脂消失，但免疫反应性糖蛋白在脑中继续表达，直到成年。坐骨神经和胚胎脑糖脂均被葡萄糖醛酸酶/硫酸酯酶处理水解，但对所有其他测试的糖苷酶不敏感。此外，观察到的4F4与提取的糖脂、糖蛋白和胚胎脑组织切片的反应性与HNK-1抗体所证明的反应性相同。在发育中的大脑的免疫细胞化学研究表明，这种碳水化合物抗原的阶段特异性分布。在E10小鼠中，免疫反应性与神经管的套层相关。在E15的皮质，最强烈的染色是与分子层和subplate，和较弱的染色被认为是在中间区和皮质板，这表明抗原是高度集中在胚胎神经系统的后迁移细胞。
Monoclonal antibody 4F4, which was raised against a cell suspension of embryonic rat forebrain, reacts with acidic glycolipids and several high-molecular-weight glycoproteins in rodent brain. The major reactive glycolipid is maximally expressed at Embryonic Day 15 (E15) and is no longer detectable at Postnatal Day 14 (P14) in the rat. 4F4 antibody reacts with a glucuronic acid- and sulfate-containing lipid isolated from human sciatic nerve as well as with lipids from mouse and rat embryonic brain tissue. Although the glycolipid disappears postnatally, the immunoreactive glycoproteins continue to be expressed in brain until adulthood. Both sciatic nerve and embryonic brain glycolipids are hydrolyzed by glucuronidase/sulfatase treatment but are insensitive to all other glycosidases tested. In addition, the observed 4F4 reactivity with extracted glycolipids, glycoproteins, and tissue sections of embryonic brain is identical to the reactivity demonstrated by HNK-1 antibodies. Immunocytochemical studies in developing brain showed stage-specific distribution of this carbohydrate antigen. At E10 in the mouse, immunoreactivity is associated with the mantle layer of the neural tube. At E15 in the cortex, the most intense staining is associated with the molecular layer and the subplate, and weaker staining is seen in the intermediate zone and cortical plate, suggesting that the antigen is highly concentrated on postmigratory cells in the embryonic nervous system.