Protective effect of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats
Protective effect of resveratrol against oxidative stress in middle cerebral artery occlusion model of stroke in rats
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DOI：
10.1016/s0024-3205(02)01691-0
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发表时间：
2002-06-28
期刊：
影响因子：
6.1
通讯作者：
Gupta, YK
中科院分区：
文献类型：
--
作者：
Sinha, K;Chaudhary, G;Gupta, YK
自由基与脑卒中缺血再灌注过程中的神经元损伤有关。反式白藜芦醇是一种有效的抗氧化剂，在葡萄和葡萄酒中发现的多酚化合物，最近在体外研究中显示出对氧化应激具有神经保护活性。在本研究中，反式白藜芦醇的慢性治疗的效果进行了评估局灶性脑缺血引起的大脑中动脉[MCA]闭塞大鼠。雄性Wistar大鼠腹腔注射反式白藜芦醇20 mg/kg，持续21天，并通过使用管腔内线闭塞MCA进行局灶性缺血。MCA闭塞两小时后，通过回撤线允许再灌注。24小时后评估动物的运动表现，随后处死大鼠以估计氧化应激标志物[丙二醛[MDA]和还原型谷胱甘肽]并评价梗死体积。对照组接受媒介物，并遵循类似的方案。在溶剂处理的MCA闭塞大鼠中观察到显著的运动障碍，MDA和还原型谷胱甘肽水平升高。与对照组相比，用反式白藜芦醇治疗可预防运动障碍、MDA和还原型谷胱甘肽水平升高，并显著降低梗死体积。这项研究提供了第一个证据，证明反式白藜芦醇在局灶性缺血中的有效性，最有可能是由于其抗氧化特性。(C)2002年由Elsevier Science Inc.出版
Free radicals have been implicated in neuronal injury during ischemia reperfusion in stroke. Trans resveratrol, a potent antioxidant, polyphenolic compound found in grapes and wines has recently been shown to have neuroprotective activity against oxidative stress in invitro studies. In the present study the effect of chronic treatment of trans resveratrol was evaluated in focal ischemia induced by middle cerebral artery [MCA] occlusion in rats. Male Wistar rats were pretreated with trans resveratrol 20 mg/kg i.p. for 21 days and were subjected to focal ischemia by occlusion of MCA using intraluminal thread. After two hours of MCA occlusion reperfusion was allowed by retracting the thread. Animals were assessed for motor performance after 24 hours and subsequently rats were sacrificed for estimation of markers of oxidative stress [malondialdehyde [MDA] and reduced glutathione] and for evaluation of volume of infarction. Control group received vehicle and similar protocol was followed. Significant motor impairment, with elevated levels of MDA and reduced glutathione was observed in the vehicle treated MCA occluded rats. Treatment with trans resveratrol prevented motor impairment, rise in levels of MDA and reduced glutathione and also significantly decreased the volume of infarct as compared to control. The study provides first evidence of effectiveness of trans resveratrol in focal ischemia most probably by virtue of its antioxidant property. (C) 2002 Published by Elsevier Science Inc.