In vivo tracking of human mesenchymal stem cells in experimental stroke
In vivo tracking of human mesenchymal stem cells in experimental stroke
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DOI：
10.3727/000000007783472381
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发表时间：
2007-01-01
影响因子：
3.3
通讯作者：
Song, Jihwan
中科院分区：
文献类型：
--
作者：
Kim, Daehong;Chun, Byoung-Gi;Song, Jihwan
为了解人骨髓间充质干细胞(HMSCs)移植到大鼠大脑中动脉闭塞(MCAO)模型后的命运，采用磁共振成像(MRI)技术。移植后10周，用亚铁氧化物(Feridex@)-鱼精蛋白硫酸盐复合体标记hMSCs，并对其进行MRI检查。组织学方法进一步证实移植细胞向梗死区的迁移。我们发现在MCAO模型中移植的hMSCs具有在同侧和对侧注射中广泛迁移到梗死区的能力，表现出致病作用。我们还分析了移植的hMSCs的详细迁移模式。我们推测，hMSCs广泛的迁移能力可能代表了开发有效的细胞移植策略治疗中风的潜力。
To understand the fates of human mesenchymal stem cells (hMSCs) following transplantation into a rodent model of middle cerebral artery occlusion (MCAo), magnetic resonance imaging (MRI) techniques were employed. hMSCs were labeled with ferumoxides (Feridex@)-protamine sulfate complexes, which were visualized and examined by MRI up to 10 weeks following transplantation. Migration of the transplanted cells to the infarcted area was further confirmed by histological methods. We found that the hMSCs transplanted in MCAo models possess the capacity to migrate to the infarcted area extensively in both ipsilateral and contralateral injections, exhibiting a pathotropism. We also analyzed the detailed migration patterns of transplanted hMSCs. We speculate that the extensive migratory ability of hMSCs may represent a therapeutic potential for developing efficient cell transplantation strategies in stroke.