Enhancing effect of low dose cyclophosphamide treatment on the in vitro antibody response
Enhancing effect of low dose cyclophosphamide treatment on the in vitro antibody response
复制标题
低剂量环磷酰胺治疗对体外抗体反应的增强作用
DOI：
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发表时间：
1977
影响因子：
5.4
通讯作者：
J. Dormont
中科院分区：
文献类型：
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作者：
H. Duclos;P. Galanaud;O. Devinsky;M. Maillot;J. Dormont
我们研究了环磷酰胺（CY）给药对小鼠随后的体外抗体应答的影响。在培养前4天用低剂量（20 mg/kg）CY处理导致对T非依赖性抗原三硝基苯基化聚丙烯酰胺（TNP-PAA）的IgM应答增加，而不影响未刺激培养物的背景应答。这表明CY处理消除了参与调节体外B细胞应答的短寿命抑制细胞。相反，相同的方案降低了裸鼠脾细胞对TNP-PAA的反应能力，表明CY-增强效应的靶点是成熟T细胞。在常规小鼠中观察到的增加的反应应该是CY对B细胞的直接抑制作用和抑制性T细胞的消除之间的平衡的结果，后一种现象在我们的条件下具有主要意义。CY增强作用的目标是不粘附塑料，但粘附Sephadx G-10柱。
We have studied the effect of cyclophosphamide (CY) administration on the subsequent in vitro antibody response in the mouse. Treatment with a low dose (20 mg/kg) of CY four days before culture results in an increased IgM response to the T‐independent antigen trinitrophenylated polyacrylamide (TNP‐PAA), without affecting the background response of unstimulated cultures. This suggests that CY treatment eliminates a short‐lived suppressor cell, involved in the regulation of the in vitro B cell response. In contrast, the same regimen decreases the ability of nude mouse spleen cells to respond to TNP‐PAA, showing that the target of CY‐enhancing effect is a mature T cell. The increased response observed in conventional mice should be the result of a balance between the direct suppressive effect of CY on B cells and the elimination of a suppressor T cell, the latter phenomenon being of predominant significance in our conditions. The target of CY‐enhancing effect is nonadherent to plastic, but adherent to Sephadx G‐10 columns.