Molecular cloning and epigenetic change detection of Kiss1 during seasonal reproduction in Chinese indigenous sheep
Molecular cloning and epigenetic change detection of Kiss1 during seasonal reproduction in Chinese indigenous sheep
复制标题
中国土羊季节繁殖过程中Kiss1的分子克隆及表观遗传变化检测
DOI：
10.1071/rd17028
复制
发表时间：
2018-04-01
影响因子：
1.9
通讯作者：
Chu, Mingxing
中科院分区：
文献类型：
--
作者：
He, Xiaoyun;Liu, Qiuyue;Chu, Mingxing
像大多数季节性驯化物种一样，绵羊是短日照繁殖者，这意味着繁殖轴由短日照激活。年光周期通过调节下丘脑促性腺激素释放激素的脉冲式分泌，影响传递到下丘脑-垂体-性腺（HPG）轴的日长信息量。Kiss 1编码的Kisspeptin在生殖季节性中起着重要作用。基于我们之前从谭（T）和小尾寒羊（STH）在乏情期和繁殖季节获得的Solexa测序数据的结果，获得了绵羊Kiss 1的全长mRNA信息; T羊为894 bp，STH羊为1145 bp。两者都编码135个氨基酸。此外，T和STH绵羊具有不同的Kiss 1转录起始位点。Kiss 1在发情期的表达量显著高于间情期（P < 0.01）。我们还发现了一个很强的关系Kiss 1 mRNA水平和组蛋白H3乙酰化状态在绵羊Kiss 1的50个启动子区域。这些数据表明，表观遗传修饰发生在繁殖过程中的绵羊，这是第一个报告，组蛋白H3脱乙酰化发生在下丘脑的季节性绵羊育种从间情期到发情期的过渡。
Like most seasonal domesticated species, sheep are short-day breeders, which means that the reproduction axis is activated by short days. The annual photoperiodic cycle affects the amount of daylength information that is transmitted to the hypothalamic-pituitary-gonadal (HPG) axis by regulating pulsatile secretion of gonadotrophin-releasing hormone from the hypothalamus. Kisspeptin, which is encoded by Kiss1, plays a major role in reproductive seasonality. Based on results from our previous Solexa sequencing data obtained from Tan (T) and Small Tail Han (STH) sheep during anoestrus and the breeding season, full-length mRNA information for ovine Kiss1 was obtained; 894 bp in T sheep and 1145 bp in STH sheep. Both encode 135 amino acids. Additionally, T and STH sheep have different transcription start sites of Kiss1. Kiss1 expression during oestrus was significantly higher than that during dioestrus, both in T and STH sheep (P < 0.01). We also found a strong relationship between Kiss1 mRNA levels and histone H3 acetylation status in the 50 promoter region of ovine Kiss1. These data indicated that epigenetic modification occurs during reproduction in sheep, and this is the first report that histone H3 deacetylation occurs in the hypothalamus of seasonal sheep breeders during the transition from dioestrus to oestrus.