Preparation of Fluorescent Diamond Nanoparticles Stably Dispersed under a Physiological Environment through Multistep Organic Transformations
Preparation of Fluorescent Diamond Nanoparticles Stably Dispersed under a Physiological Environment through Multistep Organic Transformations
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DOI：
10.1021/cm100566v
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发表时间：
2010-06-08
影响因子：
8.6
通讯作者：
Komatsu, Naoki
中科院分区：
文献类型：
--
作者：
Takimoto, Tatsuya;Chano, Tokuhiro;Komatsu, Naoki
通过有机功能化赋予纳米颗粒多种功能已经引起了人们的极大兴趣，特别是在生物医学应用方面。其中，金刚石纳米粒子，即所谓的纳米金刚石（ND），因其无毒或低毒的特性，以及能够与引入的功能进行共价键合的有机特性，被认为是最好的平台之一。在这里，我们展示了 ND 表面上的多步有机转变，通过共价键逐层聚集必要的功能。我们和其他团队最近开发的溶液相 H-1 NMR 和 C-13 NMR 光谱以及传统的红外 (IR)、荧光显微镜和光谱以及元素分析充分表征了 ND 表面引入的功能。结果，通过添加聚乙二醇（PEG）和荧光素片段，将亲水性和荧光特性结合到ND表面，从而提供在生理环境下稳定分散的荧光ND。通过将 ND 引入 HeLa 细胞，已证实其可用作细胞成像剂。
Imparting multiple functions to nanoparticles through organic functionalization has been attracting significant interest, particularly in terms of biomedical applications. Among them, diamond nanoparticles-the so-called nanodiamond (ND)-have been recognized as one of the best platforms, because of its nontoxic or low-toxicity properties, as well as its organic characteristics that enable covalent bonding with introduced functionalities. Here, we show multistep organic transformations on the ND surface that amass the requisite functions layer by layer through covalent bonds. The functionalities introduced unto the ND surface were well-characterized by solution-phase H-1 NMR and C-13 NMR spectroscopies, recently developed by us and other groups, as well as conventional infrared (IR), fluorescence microscopy and spectroscopy, and elemental analysis. As a result, hydrophilic and fluorescent characteristics were incorporated onto the ND surface by adding polyethylene glycol (PEG) and fluorescein segments, providing fluorescent ND stably dispersed under a physiological environment. ND has been confirmed to be viable for use as a cellular imaging agent by introducing them into HeLa cells.