Cyanidioschyzon merolae genome.: A tool for facilitating comparable studies on organelle biogenesis in photosynthetic eukaryotes
Cyanidioschyzon merolae genome.: A tool for facilitating comparable studies on organelle biogenesis in photosynthetic eukaryotes
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DOI：
10.1104/pp.104.053991
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发表时间：
2005-02-01
期刊：
影响因子：
7.4
通讯作者：
Kuroiwa, T
中科院分区：
文献类型：
--
作者：
Misumi, O;Matsuzaki, M;Kuroiwa, T
超小的单细胞红蓝细菌Cyanidioschyzon merolae生活在酸性温泉的极端环境中，被认为保留了细胞和基因组组织的原始特征。测定了16.5Mb的C. merolae 10D是第一个完整的藻类基因组。BLAST和注释结果表明，C. merolae具有植物和动物的混合基因库，也意味着与原核生物的关系，尽管其光合成分与其他光养生物相当。单细胞绿色衣藻已被用作光合作用、运动性和有性生殖等分子生物学研究的模型系统。虽然这两种藻类都是单细胞的，但基因组大小，细胞器数量和表面结构都有显着不同。在此，我们报道了双膜和单膜结合细胞器的特征及其相关基因。merolae的基因组编码的预测蛋白质序列进行比较分析。merolae和C.莱因哈德氏菌我们研究了预测的蛋白质的双向BLASTP分析，KOG分配，和基因注释。结果表明，大多数核心生物学功能是由数量相当的orthopathy蛋白进行的。虽然基本基因组织彼此相似，但染色质组织、细胞骨架成分和鞭毛运动的基因在C.莱因哈德氏菌分子系统发育分析表明，该微管蛋白与植物微管蛋白的亲缘关系较近，而与动物和真菌微管蛋白的亲缘关系较远。这些结果反映了C.莱因哈德氏菌
The ultrasmall unicellular red alga Cyanidioschyzon merolae lives in the extreme environment of acidic hot springs and is thought to retain primitive features of cellular and genome organization. We determined the 16.5-Mb nuclear genome sequence of C. merolae 10D as the first complete algal genome. BLASTs and annotation results showed that C. merolae has a mixed gene repertoire of plants and animals, also implying a relationship with prokaryotes, although its photosynthetic components were comparable to other phototrophs. The unicellular green alga Chlamydomonas reinhardtii has been used as a model system for molecular biology research on, for example, photosynthesis, motility, and sexual reproduction. Though both algae are unicellular, the genome size, number of organelles, and surface structures are remarkably different. Here, we report the characteristics of double membrane- and single membrane-bound organelles and their related genes in C. merolae and conduct comparative analyses of predicted protein sequences encoded by the genomes of C. merolae and C. reinhardtii. We examine the predicted proteins of both algae by reciprocal BLASTP analysis, KOG assignment, and gene annotation. The results suggest that most core biological functions are carried out by orthologous proteins that occur in comparable numbers. Although the fundamental gene organizations resembled each other, the genes for organization of chromatin, cytoskeletal components, and flagellar movement remarkably increased in C. reinhardtii. Molecular phylogenetic analyses suggested that the tubulin is close to plant tubulin rather than that of animals and fungi. These results reflect the increase in genome size, the acquisition of complicated cellular structures, and kinematic devices in C. reinhardtii.