Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus
Sexually dimorphic and ontogenetic expression of dmrt1, cyp19a1a and cyp19a1b in Gobiocypris rarus
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稀有鲫鱼中 dmrt1、cyp19a1a 和 cyp19a1b 的性别二态性和个体发育表达
DOI：
10.1016/j.cbpa.2012.03.021
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发表时间：
2012-08-01
影响因子：
2.3
通讯作者：
Zhao, Haobin
中科院分区：
文献类型：
--
作者：
Cao, Mengxi;Duan, Jundan;Zhao, Haobin
鱼类具有多样的性别决定和分化。DMRT 1和芳香化酶在门中是保守的，并在性别发育中发挥关键作用。Gobiocypris rams是一种小型鱼类，在中国被用作水生毒理学的模式，并已被用于研究环境内分泌干扰物对基因表达的影响，但其性发育仍然是难以捉摸的。本文报道了G.公羊dmrt 1及其沿着cyp19 a1 a和cyp19 a1 b的表达，cyp19 a1 a和cyp19 a1 b是两个编码性腺和脑型芳香化酶的基因，在成年和个体发育期间。cyp19a1a和dmrt1在卵巢和精巢中均有表达，但表现出性二型性。cyp19a1a在卵巢中的表达高于睾丸，而dmrt 1的表达则相反。孵化后15日龄幼鱼性腺组织学变化。dmrt1和cyp19a1a的双态表达出现在出壳后5天，早于组织学变化。CYP 19A1b与CYP 19A1a协同表达，直到孵化后15天。这些结果表明，dmrt 1和cyp19a1a基因在两性分化和性别决定中起重要作用。公羊。(C)2012 Elsevier Inc. All rights reserved.
Fish have diverse sex determination and differentiation. DMRT1 and aromatase are conserved in the phyla and play pivotal roles in sex development. Gobiocypris rams is a small fish used as a model in aquatic toxicology in China and has been used to study the effects of environmental endocrine disruptors on gene expression, but its sexual development remains elusive. Here, we report the full-length cDNA of G. rams dmrt1 and its expression along with the expression of cyp19a1a and cyp19a1b, two genes encoding gonad and brain type aromatases, in adults and during ontogenesis. Both cyp19a1a and dmrt1 are expressed in the ovary and testis but show sexual dimorphism. Expression of cyp19a1a in the ovary is higher than in testes and dmrt1 follows the opposite pattern. Juvenile gonad histology changes at 15 days after hatching. The dimorphic expression of dmrt1 and cyp19a1a appears from 5 days after hatching, which is earlier than histological change. cyp19a1b is expressed coordinately with cyp19a1a until 15 days after hatching. These results show that dmrt1 and cyp19a1a play important roles in sex determination and sex differentiation in G. rams. (C) 2012 Elsevier Inc. All rights reserved.