Assignment of a human melanoma associated gene MG50 (D2S448) to chromosome 2p25.3 by fluorescence in situ hybridization.
Assignment of a human melanoma associated gene MG50 (D2S448) to chromosome 2p25.3 by fluorescence in situ hybridization.
复制标题
通过荧光原位杂交将人类黑色素瘤相关基因 MG50 (D2S448) 分配到染色体 2p25.3。
DOI：
10.1006/geno.1994.1374
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发表时间：
1994
期刊：
影响因子：
4.4
通讯作者：
Trent,JM
中科院分区：
文献类型：
--
作者：
Weiler,SR;Taylor,SM;Deans,RJ;Kan-Mitchell,J;Mitchell,MS;Trent,JM
为了鉴定编码该基因的染色体位点，将 1 {μg}g 人类基因组克隆 (G23MG5)（其大小为 17.25-kb，包含至少 2 个对应于 D2S448 的外显子）用生物素标记，并如前所述与人类中期染色体杂交。总共检查了 44 个中期细胞，其中 42 个具有双荧光信号，2 号染色体远端短臂上的每个染色单体上都有一个荧光信号。
To identify the chromosomal locus encoding this gene, 1 {mu}g of a human genomic clone (G23MG5), which is 17.25-kb and contains at least 2 exons corresponding to D2S448, was labeled with biotin and hybridized to human metaphases chromosomes as previously described. A total of 44 metaphase cells were examined, and 42 of these had double fluorescent signals, one on each chromatid over the distal short arm of chromosome 2.