Hydrophilic interaction and reversed-phase ultra-performance liquid chromatography TOF-MS for metabonomic analysis of Zucker rat urine
Hydrophilic interaction and reversed-phase ultra-performance liquid chromatography TOF-MS for metabonomic analysis of Zucker rat urine
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DOI：
10.1002/jssc.200700644
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发表时间：
2008-05-01
影响因子：
3.1
通讯作者：
Wilson, Ian D.
中科院分区：
文献类型：
--
作者：
Gika, Helen G.;Theodoridis, Georgios A.;Wilson, Ian D.
亲水相互作用色谱法（HILIC）为RP方法提供了一种补充技术，用于基于LC-MS的代谢组学研究中极性分析物的保留。结合RP和HILIC分离的优点，通过最近引入的超高效液相色谱（UPLC）使用亚2 μ m颗粒获得的高效快速分离，能够实现生物样品中存在的代谢物的覆盖率增加。本文开发了HILIC-UPLC-MS方法，以提供从雄性Zucker大鼠获得的尿样的代谢产物谱。将所得数据与通过RP-UPLC-MS获得的相同样品的结果进行比较，并证明了两种方法的两种分离的互补性，从而能够区分不同的样品类型。有趣的是，样品类型区分是基于不同的标记物。
Hydrophilic interaction chromatography (HILIC) provides a complementary technique to RP methods for the retention of polar analytes for LC-MS-based metabonomic studies. Combining the advantages of both RP and HILIC separations with the efficient and rapid separations obtained using sub-2 mu m particles via the recently introduced ultra-performance LC (UPLC) enables increased coverage of the metabolites present in biological samples to be achieved. Here an HILIC-UPLC-MS method was developed to provide metabolite profiles for urine samples obtained from male Zucker rats. The resulting data were compared with results obtained for the same samples by RP-UPLC-MS and demonstrated the complementary nature of the two separations with both methods enabling discrimination between the different sample types. Interestingly sample type differentiation was based on different markers.