Peroxynitrite inhibits Ca2+-activated K+ channel activity in smooth muscle of human coronary arterioles
Peroxynitrite inhibits Ca2+-activated K+ channel activity in smooth muscle of human coronary arterioles
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DOI：
10.1161/01.res.0000046003.14031.98
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发表时间：
2002-11-29
影响因子：
20.1
通讯作者：
Gutterman, DD
中科院分区：
文献类型：
--
作者：
Liu, YP;Terata, K;Gutterman, DD
我们检验了ONOO-，超氧阴离子（O-2（.-））和一氧化氮（NO）抑制人冠状小动脉（HCA）的血管平滑肌细胞（VSMC）中钙激活的K+（Ka（Ca））通道活性，从而降低超极化介导的血管舒张。从右心耳解剖HCA。ONOO-与微血管的相互作用通过使用硝基酪氨酸抗体的免疫组化来确定。在暴露于真实ONOO-或硝普钠（SNP）+黄嘌呤（XA）+黄嘌呤氧化酶（XO）的动脉中观察到强染色。ONOO-（-2.5 +/- 8%; P
We examined the hypothesis that ONOO-, a product of the interaction between superoxide (O-2(.-)) and nitric oxide (NO), inhibits calcium-activated K+ (Ka(Ca)) channel activity in vascular smooth muscle cells (VSMCs) of human coronary arterioles (HCAs), thereby reducing hyperpolarization-mediated vasodilation. HCAs were dissected from right atrial appendages. The interaction of ONOO- with rnicrovessels was determined by immunochistochemistry using a nitrotyrosine antibody. Strong staining was observed in arteries exposed to authentic ONOO- or to sodium nitroprusside (SNP)+xanthine (XA)+xanthine oxidase (XO). Dilation to 10(-8) mol/L bradykinin (BK) was abolished in vessels exposed to ONOO- (-2.5 +/- 8%; P