Gut microbiota and development of atopic eczema in 3 European birth cohorts
Gut microbiota and development of atopic eczema in 3 European birth cohorts
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DOI：
10.1016/j.jaci.2007.05.018
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发表时间：
2007-08-01
影响因子：
14.2
通讯作者：
Wold, Agnes E.
中科院分区：
文献类型：
--
作者：
Adlerberth, Ingegerd;Strachan, David P.;Wold, Agnes E.
背景资料：肠道微生物的免疫系统的刺激可能会阻止过敏development.Objective：本研究探讨了这一假设，即食物过敏原和特应性湿疹的敏感性的婴儿肠道定植pattern.Methods：婴儿围产期在哥德堡（n = 116），伦敦（n = 108），和罗马（n = 100）招募。在直肠（3天）和定量粪便培养物（7、14和28天以及2、6和12个月龄）中鉴定了属或种水平的共生细菌。在18个月的年龄，特应性湿疹和总的和食物特异性IgE水平进行了评估。这些结果与11个细菌群的定植时间以及在某些时间点严格厌氧菌与兼性厌氧菌和革兰氏阳性菌与革兰氏阴性菌的比率相关。研究中心，分娩方式，产次和婴儿饮食被纳入作为协变量。结果：无论是特应性湿疹，也不是食物特异性IgE的18个月的年龄与时间的收购任何特定的细菌组。剖腹产延迟了大肠杆菌、拟杆菌和双歧杆菌的定植，让位于梭菌等。缺乏年长的兄弟姐妹与梭菌属物种的早期定植和12个月时较低的严格厌氧/兼性厌氧比率有关。结论：这项研究不支持欧洲婴儿早期对食物或特应性湿疹的敏感性与缺乏任何特定的可培养肠道细菌有关的假设。临床意义：防止过敏所需的微生物刺激的性质仍有待确定。
Background: Stimulation of the immune system by gut microbes might prevent allergy development.Objective: The present study examined the hypothesis that sensitization to food allergens and atopic eczema are influenced by the infantile intestinal colonization pattern.Methods: Infants were recruited perinatally in Goteborg (n = 116), London (n = 108), and Rome (n = 100). Commensal bacteria were identified to the genus or species level in rectal (3 days) and quantitative stool cultures (7, 14, and 28 days and 2, 6, and 12 months of age). At 18 months of age, atopic eczema and total and food-specific IgE levels were assessed. These outcomes were modeled in relation to time to colonization with 11 bacterial groups and to ratios of strict anaerobic to facultative anaerobic bacteria and gram-positive to gram-negative bacteria at certain time points. Study center, mode of delivery, parity, and infant diet were included as covariates.Results: Neither atopic eczema nor food-specific IgE by 18 months of age were associated with time of acquisition of any particular bacterial group. Cesarean section delayed colonization by Escherichia coli and Bacteroides and Bifidobacterium species, giving way to, for example, Clostridium species. Lack of older siblings was associated with earlier colonization by Clostridium species and lower strict anaerobic/facultative anaerobic ratio at 12 months.Conclusions: This study does not support the hypothesis that sensitization to foods or atopic eczema in European infants in early life is associated with lack of any particular culturable intestinal commensal bacteria.Clinical implications: The nature of the microbial stimulus required for protection from allergy remains to be identified.