Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling.
Positioning of centrioles is a conserved readout of Frizzled planar cell polarity signalling.
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DOI：
10.1038/ncomms11135
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发表时间：
2016-03-29
影响因子：
16.6
通讯作者：
Mlodzik M
中科院分区：
文献类型：
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作者：
Carvajal-Gonzalez JM;Roman AC;Mlodzik M
平面细胞极性（PCP）信号传导是一个保守的发育途径，在发育过程中调节细胞的方向。进化上保守的通路读出尚未建立，此外，认为PCP介导的细胞应答是组织特异性的。五氯苯酚在脊椎动物中的一个关键功能是调节协调的中心粒/纤毛定位，这一功能与果蝇中的五氯苯酚无关。在这里，我们报告指导性的卷曲-PCP（FZ/PCP）信号进入极化中心粒定位在果蝇翅膀。我们发现，中心粒极化蛹翅细胞作为PCP信号的读出，与增益和功能丧失FZ/PCP信号影响中心粒极化。重要的是，中心粒的丢失或获得不影响Fz/PCP的建立，暗示中心粒定位为保守的PCP读数，可能是PCP调节的肌动蛋白聚合的下游。连同脊椎动物的数据，这些结果表明一个统一的模型，中心粒/纤毛定位作为一个共同的下游效应的五氯苯酚信号从苍蝇到哺乳动物。 平面细胞极性（PCP）在发育过程中有助于细胞定向，但这在果蝇中是如何调节的尚不清楚。在这里，作者确定卷曲PCP信号调节极化中心粒定位在翼盘。
Planar cell polarity (PCP) signalling is a well-conserved developmental pathway regulating cellular orientation during development. An evolutionarily conserved pathway readout is not established and, moreover, it is thought that PCP mediated cellular responses are tissue-specific. A key PCP function in vertebrates is to regulate coordinated centriole/cilia positioning, a function that has not been associated with PCP in Drosophila. Here we report instructive input of Frizzled-PCP (Fz/PCP) signalling into polarized centriole positioning in Drosophila wings. We show that centrioles are polarized in pupal wing cells as a readout of PCP signalling, with both gain and loss-of-function Fz/PCP signalling affecting centriole polarization. Importantly, loss or gain of centrioles does not affect Fz/PCP establishment, implicating centriolar positioning as a conserved PCP-readout, likely downstream of PCP-regulated actin polymerization. Together with vertebrate data, these results suggest a unifying model of centriole/cilia positioning as a common downstream effect of PCP signalling from flies to mammals. Planar cell polarity (PCP) contributes to cellular orientation during development but how this is regulated in Drosophila is unclear. Here, the authors identify Frizzled-PCP signalling as regulating polarised centriole positioning in the wing disc.