Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy
Use of the 2A sequence from foot-and-mouth disease virus in the generation of retroviral vectors for gene therapy
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DOI：
10.1038/sj.gt.3300811
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发表时间：
1999-02-01
期刊：
影响因子：
5.1
通讯作者：
Izquierdo, M
中科院分区：
文献类型：
--
作者：
de Felipe, P;Martín, V;Izquierdo, M
我们描述了逆转录病毒载体的构建，其中两个基因由至少96个核苷酸连接，编码来自口蹄疫病毒(FMDV)的微小微小病毒(Picorna Virus)口蹄疫病毒(FMDV)的2A序列。转录和翻译产生一个双顺反子mRNA和两个独立的蛋白质产物。该系统提供了其他替代方法创建多顺反子mRNAs的优势，并可用于基因治疗递送载体。
We describe the construction of retroviral plasmid vectors in which two genes are linked by a minimum of 96 nucleotides encoding the 2A sequence from the picornavirus foot-and-mouth disease virus (FMDV). Transcription and translation gives rise to a bicistronic mRNA and two independent protein products. The system offers advantages to other alternative ways to create polycistronic mRNAs and can be used in gene therapy delivery vectors.