INDUCTION OF MEMBRANE PROLIFERATION BY POLIOVIRUS PROTEINS 2C AND 2BC
INDUCTION OF MEMBRANE PROLIFERATION BY POLIOVIRUS PROTEINS 2C AND 2BC
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DOI：
10.1006/bbrc.1995.1010
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发表时间：
1995-01-05
影响因子：
3.1
通讯作者：
CARRASCO, L
中科院分区：
文献类型：
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作者：
ALDABE, R;CARRASCO, L
脊髓灰质炎病毒感染导致出现许多参与病毒基因组复制的细胞质空泡。为了表征参与膜增殖的病毒蛋白，不同的脊髓灰质炎病毒蛋白已在HeLa细胞中表达。已经获得了两种表达脊髓灰质炎病毒蛋白2C的重组牛痘病毒，一种在脊髓灰质炎病毒的5 '非翻译（UTR）序列下，另一种在EMC病毒的前导区下。2C的表达在两种情况下都非常有效，尽管当脊髓灰质炎病毒2C在EMC病毒的5'UTR序列下表达时获得了更好的结果。使用含有噬菌体T7 RNA聚合酶的重组牛痘病毒分析置于T7启动子下的脊髓灰质炎病毒蛋白2B、2C或2BC的瞬时表达。与2B相反，2C或2BC的表达能够诱导形态上类似于脊髓灰质炎病毒感染期间发现的空泡的增殖。这些发现表明，脊髓灰质炎病毒蛋白2C，除了其NTR和RNA结合活性，也被赋予了诱导细胞质空泡形成的能力。(C)出版社：Academic Press
Poliovirus infection leads to the appearance of a number of cytoplasmic vacuoles involved in the replication of virus genomes. To characterize the viral proteins involved in membrane proliferation different poliovirus proteins have been expressed in HeLa cells. Two recombinant vaccinia viruses have been obtained that express poliovirus protein 2C, one under the 5' untranslated (UTR) sequence of poliovirus and another under the leader region of EMC virus. Expression of 2C was very efficient in both cases, although better results were obtained when poliovirus 2C was expressed under the 5'UTR sequence of EMC virus. Transient expression of poliovirus proteins 2B, 2C or 2BC placed under a T7 promoter was analyzed using a recombinant vaccinia virus that contains the bacteriophage T7 RNA polymerase. The expression of 2C, or 2BC, contrary to 2B, was able to induce the proliferation of vacuoles morphologically similar to those found during poliovirus infection. These findings indicate that poliovirus protein 2C, in addition to its NTPase and RNA binding activities,is also endowed with the capacity to induce the formation of cytoplasmic vacuoles. (C) 1995 Academic Press, Inc.