Importance of the herpes simplex virus UL24 gene for productive ganglionic infection in mice
Importance of the herpes simplex virus UL24 gene for productive ganglionic infection in mice
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DOI：
10.1006/viro.1997.9012
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发表时间：
1998-03-01
期刊：
影响因子：
3.7
通讯作者：
Coen, DM
中科院分区：
文献类型：
--
作者：
Jacobson, JG;Chen, SH;Coen, DM
单纯疱疹病毒UL24基因与病毒胸苷激酶（tk）基因重叠。以前对UL24的大多数研究都检查了UL24突变体，这些突变体也含有tk，有时也含有其他突变。为了解决UL24在细胞培养和哺乳动物宿主感染中对病毒复制的重要性，我们构建了一种突变病毒，其中包含UL24无义突变，不影响TK活性；另一种突变病毒包含UL24聚集点突变和TK突变，其本身不影响病毒在小鼠神经节中急性复制的能力，也不影响在小鼠角膜接种后潜伏感染后重新激活的能力。这两种突变病毒在培养细胞和小鼠眼睛中复制，尽管效率低于野生型或对照病毒。这两种突变体在三叉神经节的急性复制和三叉神经节外植体后潜伏期的再激活方面受到更严重的损害。尽管在无义突变体感染的神经节中存在较低水平的病毒DNA和潜伏期相关转录本，这些结果表明UL24对小鼠感觉神经节的生产感染特别重要，并且可能对某些tk突变体的发病机制的行为有影响。(C) 1998学术出版社。
The UL24 gene of herpes simplex virus overlaps the viral thymidine kinase (tk) gene. Most previous studies of UL24 have examined UL24 mutants that have also contained tk and sometimes other mutations. To address the importance of UL24 for viral replication in cell culture and in infections of a mammalian host, we constructed a mutant virus containing a UL24 nonsense mutation that does not affect TK activity and a second mutant that contains clustered point mutations in UL24 and a mutation in tk that does not by itself affect the ability of the virus to replicate acutely in mouse ganglia or to reactivate from latent infection following corneal inoculation of mice. Both mutant viruses replicated in cells in culture and in the mouse eye, albeit less efficiently than wild type or control viruses. Both mutants were much more severely impaired for acute replication in trigeminal ganglia and for reactivation from latency following explant of these ganglia. Viral DNA and latency-associated transcripts were present, albeit at lower levels in ganglia infected with the nonsense mutant These results indicate that UL24 is especially important for productive infection of mouse sensory ganglia and may have implications for the behaviors of certain tk mutants in pathogenesis. (C) 1998 Academic Press.