Measurement of cell volume loss in the liquid region preceding an advancing phase change interface
Measurement of cell volume loss in the liquid region preceding an advancing phase change interface
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DOI：
10.1111/j.1749-6632.1998.tb10162.x
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发表时间：
1998-01-01
期刊：
影响因子：
--
通讯作者：
Brand, JJ
中科院分区：
文献类型：
--
作者：
Harmison, HR;Diller, KR;Brand, JJ
众所周知，溶液的凝固导致溶质在液体区中的重新分布。对于细胞悬浮液的冷冻，预期溶质在导致生长的冰相的区域中的积累将在冰相到达细胞之前在细胞中引起渗透反应。为了测量特定藻类中的这种现象，利用定向凝固低温显微镜研究了冷冻过程中得克萨斯绿球藻的体积变化，当细胞遇到移动相前沿时，相对细胞体积作为温度和位置的函数被连续跟踪。在生长的固相之前的含有浓缩溶质的液体区域中测量细胞体积的损失。
It is well understood that the solidification of a solution results in a redistribution of solute in the liquid zone. For the freezing of suspensions of cells it is anticipated that accumulation of solute in the region leading a growing ice phase will cause an osmotic response in cells before the ice phase reaches the cells. To measure this phenomenon in a specific algal species, the volume changes in Chlorococcum texanum during freezing were studied using directional solidification cryomicroscopy, The relative cell volume was tracked continuously as a function of temperature and position as cells encountered the moving phase front. The loss of cell volume was measured in the liquid region containing concentrated solute ahead of the growing solid phase.