Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination
Deep Imaging Analysis in VISUAL Reveals the Role of YABBY Genes in Vascular Stem Cell Fate Determination
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DOI：
10.1093/pcp/pcaa002
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发表时间：
2020-02-01
影响因子：
4.9
通讯作者：
Kondo, Yuki
中科院分区：
文献类型：
--
作者：
Nurani, Alif Meem;Ozawa, Yasuko;Kondo, Yuki
干细胞经历细胞分裂和分化以确保组织发育。由于植物细胞是固定的，植物干细胞应该在分化之前决定它们的细胞命运，将特化细胞定位在正确的位置。在这项研究中，基于化学筛选，我们分离出一种新的次生细胞壁指示剂BF-170，它结合到木质素，可用于图像在体外和原位木质部的发展。使用BF-170观察维管分化模式在体外维管细胞诱导系统，VISUAL，显示近轴叶肉细胞的子叶主要产生异位木质部细胞。此外，韧皮部细胞大量产生的远轴层，表明参与叶近轴-远轴极性在决定维管细胞的命运。远轴极性突变体的分析突出了YAB 3，一个远轴细胞命运调节器，在抑制木质部和促进韧皮部分化的远轴域在VISUAL的作用。此外，YABBY家族基因影响体内次生生长过程中的血管发育。我们的研究结果表明，这种介质的空间信息有助于正确地确定血管干细胞的细胞命运的可能性，以保护陆地植物的血管模式。
Stem cells undergo cell division and differentiation to ensure organized tissue development. Because plant cells are immobile, plant stem cells ought to decide their cell fate prior to differentiation, to locate specialized cells in the correct position. In this study, based on a chemical screen, we isolated a novel secondary cell wall indicator BF-170, which binds to lignin and can be used to image in vitro and in situ xylem development. Use of BF-170 to observe the vascular differentiation pattern in the in vitro vascular cell induction system, VISUAL, revealed that adaxial mesophyll cells of cotyledons predominantly generate ectopic xylem cells. Moreover, phloem cells are abundantly produced on the abaxial layer, suggesting the involvement of leaf adaxial-abaxial polarity in determining vascular cell fate. Analysis of abaxial polarity mutants highlighted the role of YAB3, an abaxial cell fate regulator, in suppressing xylem and promoting phloem differentiation on the abaxial domains in VISUAL. Furthermore, YABBY family genes affected in vivo vascular development during the secondary growth. Our results denoted the possibility that such mediators of spatial information contribute to correctly determine the cell fate of vascular stem cells, to conserve the vascular pattern of land plants.