Genetic dissection of meiotic cytokinesis in Drosophila males
Genetic dissection of meiotic cytokinesis in Drosophila males
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DOI：
10.1091/mbc.e03-08-0603
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发表时间：
2004-05-01
影响因子：
3.3
通讯作者：
Gatti, M
中科院分区：
文献类型：
--
作者：
Giansanti, MG;Farkas, RM;Gatti, M
我们用果蝇雄性减数分裂作为胞质分裂机制遗传解剖的模型系统。果蝇减数分裂胞质分裂缺陷突变体可以通过其多核精子细胞容易地鉴定。此外，大尺寸的减数分裂纺锤体允许突变体表型的特征与精致的细胞学分辨率。我们筛选了1955个纯合突变雄性不育系中的多核精子细胞，从而确定了胞质分裂所需的19个基因的突变。这些包括16个新的基因座和三个基因，透明，四轮驱动，卵石，已经知道参与果蝇胞质分裂。为了确定导致胞质分裂失败的主要缺陷，我们分析了这19个基因中每一个的雄性突变体的减数分裂。微管蛋白，苯胺，KLP 3A，和F-肌动蛋白染色的制剂的检查显示离散的缺陷，在组件的细胞动力学装置，这表明这些基因的作用在四个主要点在一个逐步的胞质分裂途径。我们的研究结果还表明，中央纺锤体和收缩环是相互依赖的结构，在整个胞质分裂相互作用。此外，我们的遗传和细胞学分析提供了进一步的证据，果蝇胞质分裂的细胞类型特异性控制，这表明，在男性减数分裂胞质分裂所需的几个基因是不需要的有丝分裂胞质分裂。
We have used Drosophila male meiosis as a model system for genetic dissection of the cytokinesis mechanism. Drosophila mutants defective in meiotic cytokinesis can be easily identified by their multinucleate spermatids. Moreover, the large size of meiotic spindles allows characterization of mutant phenotypes with exquisite cytological resolution. We have screened a collection of 1955 homozygous mutant male sterile lines for those with multinucleate spermatids, and thereby identified mutations in 19 genes required for cytokinesis. These include 16 novel loci and three genes, diaphanous, four wheel drive, and pebble, already known to be involved in Drosophila cytokinesis. To define the primary defects leading to failure of cytokinesis, we analyzed meiotic divisions in male mutants for each of these 19 genes. Examination of preparations stained for tubulin, anillin, KLP3A, and F-actin revealed discrete defects in the components of the cytokinetic apparatus, suggesting that these genes act at four major points in a stepwise pathway for cytokinesis. Our results also indicated that the central spindle and the contractile ring are interdependent structures that interact throughout cytokinesis. Moreover, our genetic and cytological analyses provide further evidence for a cell type-specific control of Drosophila cytokinesis, suggesting that several genes required for meiotic cytokinesis in males are not required for mitotic cytokinesis.