A NEW LOOK AT MUMPS ENCEPHALITIS: INCLUSION BODIES AND CYTOPATHIC EFFECTS
A NEW LOOK AT MUMPS ENCEPHALITIS: INCLUSION BODIES AND CYTOPATHIC EFFECTS
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腮腺炎脑炎的新视角：包涵体和细胞病变效应
DOI：
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发表时间：
1974
影响因子：
3.2
通讯作者：
J. Baringer
中科院分区：
文献类型：
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作者：
G. Margolis;L. Kilham;J. Baringer
约翰逊和约翰逊最近的研究为腮腺炎并发脑炎和腮腺炎病毒对发育中的大脑的致病性提供了新的见解。接种到乳仓鼠体内后，腮腺炎病毒会诱发破坏性室管膜炎，治愈后会导致阻塞性脑积水。然而，即使是神经适应性菌株的代理，已被描述为非神经细胞病变，尽管在神经元中广泛复制的能力。典型的细胞质内含物诱导的组织培养细胞，但从来没有承认在神经组织中的内含物，尽管突出的特定颗粒免疫荧光室管膜和神经元。我们已经在乳仓鼠中使用神经病病毒株进行了研究，并采用了固定和染色方法，这些方法被证明是解决这些差异的神经病毒学研究的上级方法。在神经元、室管膜和脉络丛中观察到广泛的胞质内含物。这些夹杂物具有相同的分布抗原所示的免疫荧光和病毒聚集体显示的电子显微镜，但更少，更广泛。相关的细胞病变效应在室管膜中最为突出，但也存在于神经元中，特别是在海马中。
Recent studies by Johnson and Johnson have provided new insights into encephalitis complicating mumps and the pathogenicity of mumps virus for the developing brain. Upon inoculation into suckling hamsters mumps virus induces a destructive ependymitis which, upon healing, leads to an obstructive hydrocephalus. Even neuroadapted strains of the agent, however, have been described as non-neurocytopathic, despite the ability to replicate widely in neurons. Typical cytoplasmic inclusions are induced in tissue culture cells, but inclusions have never been recognized in neural tissues, despite the prominence of specific granular immuno fluorescence in ependyma and neurons. We have carried out studies in suckling hamsters using a neuropathic strain of virus and employing fixation and staining methods proven superior for neurovirologic studies to resolve these discrepancies. Widespread cytoplasmic inclusions were observed in neurons, ependyma, and choroid plexus. These inclusions had the same distribution as antigen demonstrated by immunofluorescence and of virus aggregates revealed by electron microscopy, but were fewer and less widespread. Associated cytopathic effects were most prominent in ependyma, but were also present in neurons, particularly in the hippocampus.