Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses
Comparative analysis of budded virus infectivity of Bombyx mandarina and B. mori nucleopolyhedroviruses
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DOI：
10.1007/s11262-011-0623-7
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发表时间：
2011-10-01
期刊：
影响因子：
1.6
通讯作者：
Katsuma, Susumu
中科院分区：
文献类型：
--
作者：
Kokusho, Ryuhei;Zhang, Chuan-Xi;Katsuma, Susumu
家蚕核型多角体病毒（BomaNPV）是家蚕核型多角体病毒（BmNPV）的一种变异体。BomaNPV S1株经口感染B的毒力明显低于BmNPV T3株。家蚕幼虫，但S1的其他功能，包括出芽病毒（BV）的感染性和病毒在培养细胞中的繁殖仍然是未知的。本研究比较了B中S1和T3的BV感染性。家蚕幼虫和培养细胞。通过血腔内BV注射进行的幼虫生物测定显示，S1的BV的半数致死剂量比T3的低约3倍。此外，S1在B的血淋巴中产生了更多的BV和闭塞体（OB）。家蚕幼虫与T3.此外，我们还观察到，与T3感染的幼虫相比，S1感染的幼虫的运动增强更早，半数致死时间更短。S1和T3感染的BmN细胞的Western印迹分析显示，S1感染的细胞中晚期和极晚期基因产物的表达高于T3感染的细胞。总的来说，这些结果清楚地表明，在两个B中，S1的BV感染性高于T3。家蚕幼虫和培养的细胞，虽然S1的OBs对B的感染性要小得多。家蚕幼虫的生长量高于T3。
Bombyx mandarina nucleopolyhedrovirus (BomaNPV) is a variant of Bombyx mori nucleopolyhedrovirus (BmNPV). BomaNPV S1 strain has been reported to be significantly less virulent than the BmNPV T3 strain via the oral infection route in B. mori larvae, but other features of S1 including budded virus (BV) infectivity and virus propagation in cultured cells are still unknown. In this study, we compared BV infectivity of S1 and T3 in B. mori larvae and cultured cells. Larval bioassays by intrahemocoelic BV injection revealed that the median lethal dose of S1's BV was approximately three times lower than that of T3. In addition, S1 produced more BVs and occlusion bodies (OBs) in the hemolymph of B. mori larvae compared with T3. Furthermore, we observed that the locomotion was enhanced earlier and the median lethal time was shorter in S1-infected larvae compared with those in T3-infected larvae. Western blot analysis of S1- and T3-infected BmN cells revealed that expression of late and very late gene products in S1-infected cells was higher than that in T3-infected cells. Collectively, these results clearly show that S1's BV infectivity is higher than that of T3 in both B. mori larvae and cultured cells, although S1's OBs are much less infectious to B. mori larvae than T3's.