Differential expression of microRNAs in aortic tissue and plasma in patients with acute aortic dissection.
Differential expression of microRNAs in aortic tissue and plasma in patients with acute aortic dissection.
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急性主动脉夹层患者主动脉组织和血浆中microRNA的差异表达
DOI：
10.11909/j.issn.1671-5411.2015.06.013
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发表时间：
2015-11
期刊：
影响因子：
--
通讯作者：
Hui RT
中科院分区：
文献类型：
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作者：
Wang XJ;Huang B;Yang YM;Zhang L;Su WJ;Tian L;Lu TY;Zhang S;Fan XH;Hui RT
背景生物标志物辅助诊断急性主动脉夹层（AAD）对诊断和治疗具有重要意义。然而，确定血液中AAD的生物标志物是一项具有挑战性的任务。本研究的目的是寻找AAD中新的潜在microRNA（miRNAs）生物标志物。方法采用基因芯片技术检测两组升主动脉组织和血浆中miRNAs的表达谱。组织组由4名AAD患者和4名健康男性器官供体对照组成。血浆组包括20例AAD患者和20例无心血管疾病的对照组。采用生物信息学方法分析差异表达miRNAs的潜在靶点。结果AAD患者主动脉组织中差异表达的miRNAs有30个，其中13个上调，17个下调;血浆中差异表达的miRNAs有93个，其中33个上调，60个下调。发现AAD患者的主动脉组织和血浆中有4种miRNA表达上调。预测的miRNA靶点表明，四种失调的miRNA主要靶向与细胞-细胞粘附、细胞外基质代谢、细胞骨架组织、炎症以及与细胞周期相关的多种信号通路相关的基因。结论在AAD患者的主动脉组织和血浆中发现了4种表达上调的miRNAs。这些miRNAs可能是AAD潜在的诊断生物标志物。需要进行更大规模的抽样调查以进一步验证。
Background Biomarker-assisted diagnosis of acute aortic dissection (AAD) is important for diagnosis and treatment. However, identification of biomarkers for AAD in blood is a challenging task. The aim of this study is to search for new potentially microRNA (miRNAs) biomarkers in AAD. Methods The miRNAs expression profiles in ascending aortic tissue and plasma were examined by microarray analysis in two sets or groups. The tissue group was composed of four patients with AAD and four controls of healthy male organ donors. The plasma group included 20 patients with AAD and 20 controls without cardiovascular disease. Bioinformatics was used to analyze the potential targets of the differentially expressed miRNAs. Results Our study revealed that in AAD patients, the aortic tissue had 30 differentially expressed miRNAs with 13 up-regulated and 17 down-regulated, and plasma had 93 differentially expressed miRNAs, of which 33 were up-regulated and 60 were down-regulated. Four miRNAs were found to be up-regulated in both aortic tissue and plasma in AAD patients. The predicted miRNA targets indicated the four dysregulated miRNAs mainly targeted genes that were associated with cell-cell adhesion, extracellular matrix metabolism, cytoskeleton organization, inflammation, and multiple signaling pathways related to cellular cycles. Conclusions Four miRNAs, which are up-regulated both in aortic tissue and in plasma in AAD patients, have been identified in this study. These miRNAs might be potential diagnostic biomarkers for AAD. Larger sample investigations are needed for further verification.