Toxicological Effect and Molecular Mechanism of the Chiral Neonicotinoid Dinotefuran in Honeybees
Toxicological Effect and Molecular Mechanism of the Chiral Neonicotinoid Dinotefuran in Honeybees
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手性新烟碱呋虫胺对蜜蜂的毒理学作用及分子机制
DOI：
10.1021/acs.est.1c05692
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发表时间：
2021
影响因子：
11.4
通讯作者：
Meirong Zhao
中科院分区：
文献类型：
--
作者：
Quan Zhang;Lili Fu;Tao Cang;Tao Cang;Mingcheng Guo;Bingbing Zhou;Guohua Zhu;Meirong Zhao
随着授粉服务需求的不断增加，蜜蜂的健康受到广泛关注。最近的证据表明，蜜蜂的健康可能会构成潜在的威胁，广泛使用的烟碱类化合物在世界各地。然而，这些杀虫剂在蜜蜂中的分子机制，特别是在对映体水平上知之甚少。本研究选用意大利蜜蜂（Apis mellifera，A. mellifera）和中华蜜蜂Apis cerana（A. cerana），探讨了烟碱类呋虫胺及其对映体的毒性及分子机制。结果表明，经口和接触暴露，S-呋虫胺对蜜蜂的毒性是外消旋-呋虫胺和R-呋虫胺的114倍。对高毒力的S-呋虫胺对中华按蚊的免疫系统有较强的敏感性。cerna暴露48 h后，观察到神经元信号的显著变化。三种形式的呋虫胺暴露。此外，分子对接结果还表明，S-呋虫胺与烟碱型乙酰胆碱受体形成的氢键比R-呋虫胺多，说明S-呋虫胺的毒性更强。本文提供的数据表明，R-呋虫胺可能是一种比rac-呋虫胺更安全的防治害虫和保护传粉者的替代品。
With the increasing demand for pollinating services, the wellness of honeybees has received widespread attention. Recent evidence indicated honeybee health might be posed a potential threat by widely used neonicotinoids worldwide. However, little is known about the molecular mechanism of these insecticides in honeybees especially at an enantiomeric level. In this study, we selected two species of bees,Apis mellifera(A. mellifera) andApis cerana(A. cerana), to assess the toxicity and molecular mechanism of neonicotinoid dinotefuran and its enantiomers. The results showed thatS-dinotefuran was more toxic thanrac-dinotefuran andR-dinotefuran to honeybees by oral and contact exposures as much as 114 times.A. ceranawas more susceptible to highly toxic enantiomerS-dinotefuran.S-dinotefuran induced the immune system response inA. ceranaafter 48 h exposure and significant changes were observed in the neuronal signaling ofA. melliferaunder three forms of dinotefuran exposure. Moreover, molecular docking also revealed thatS-dinotefuran formed more hydrogen bonds thanR-dinotefuran with nicotinic acetylcholine receptor, indicating the higher toxicity ofS-dinotefuran. Data provided here show thatR-dinotefuran may be a safer alternative to control pests and protect pollinators thanrac-dinotefuran.