Sesquiterpenoids with free-radical-scavenging properties from marine macroalga Ulva fasciata Delile
Sesquiterpenoids with free-radical-scavenging properties from marine macroalga Ulva fasciata Delile
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DOI：
10.1016/j.foodchem.2010.02.012
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发表时间：
2010-09-01
期刊：
影响因子：
8.8
通讯作者：
Paulraj, R.
中科院分区：
文献类型：
--
作者：
Chakraborty, Kajal;Paulraj, R.
采用1，1-二苯基-2-苦肼基（DPPH）和2，2 '-连氮基-双（3-乙基-苯并噻唑啉-6-磺酸盐）（ABTS）体外清除自由基试验，评价了印度半岛西南海岸产的一种重要的食用绿植烷类海洋植物-条带万岁（Viva fasciata）的各种溶剂提取物的自由基清除活性。在0.1 mg/ml浓度下，粉条Viva fasciata乙酸乙酯提取物对DPPH（89.8 ± 4.2%）和ABTS（+）的清除率（82.6 ± 3.7%）明显高于二氯甲烷和正己烷提取物。使用石油醚/EtOAc的阶梯梯度，自由基清除测定引导的EtOAc提取物的色谱分离产生五种主要的倍半萜类化合物。孵育8分钟后，ABTS.*其中一种倍半萜类化合物的清除活性（71.4 ± 1.5%）高于Trolox（44.1 ± 1.5%），因此可能具有作为食品工业中的天然抗氧化剂的潜力。(C)2010爱思唯尔有限公司版权所有。
Free-radical-scavenging activities of various solvent extracts of Viva fasciata, a chlorophytan marine macroalga with significance as a food ingredient, from the southwestern coast of the Indian peninsula, were evaluated using in vitro tests, including 1,1-dipheny1-2-picrylhydrazyl (DPPH) and 2,2'-azino-bis(3-ethyl-benzothiazoline-6-sulfonate) (ABTS) scavenging assays. Ethyl acetate (EtOAc) extract of Viva fasciata displayed markedly stronger DPPH (89.8 +/- 4.2%) and ABTS(+) scavenging (82.6 +/- 3.7%) activities at 0.1 mg/ml than dichloromethane and n-hexane extracts. Radical scavenging assay-guided chromatographic separation of the EtOAc extract, using a step gradient of petroleum ether/EtOAc yielded five major sesquiterpenoids. After 8 min of incubation the ABTS.* scavenging activity of one of these sesquiterpenoids was higher (71.4 1.5%) than that of Trolox (44.1 1.5%), and therefore may have potential as a natural antioxidant in the food industry. (C) 2010 Elsevier Ltd. All rights reserved.