Structures of the bacterial ribosome at 3.5 Å resolution
Structures of the bacterial ribosome at 3.5 Å resolution
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DOI：
10.1126/science.1117230
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发表时间：
2005-11-04
期刊：
影响因子：
56.9
通讯作者：
Cate, JHD
中科院分区：
文献类型：
--
作者：
Schuwirth, BS;Borovinskaya, MA;Cate, JHD
我们描述了完整的细菌核糖体的两种结构，通过X射线结晶学测定，其分辨率为3.5埃。这些结构提供了完整核糖体背景下大小核糖体亚基之间的界面和肽基转移酶中心的构象的详细视图。两个核糖体之间的差异揭示了头部和小亚基其余部分之间的高度灵活性。在本结构中观察到的小亚基头部的旋转，与先前观察到的两个亚基的棘齿状运动相结合，表明了信使RNA(MRNA)和转移RNA(TRNAs)在易位过程中的最终运动机制。
We describe two structures of the intact bacteria[ ribosome from Escherichia coli determined to a resolution of 3.5 angstroms by x-ray crystallography. These structures provide a detailed view of the interface between the small and large ribosomal subunits and the conformation of the peptidyl transferase center in the context of the intact ribosome. Differences between the two ribosomes reveal a high degree of flexibility between the head and the rest of the small subunit. Swiveling of the head of the small subunit observed in the present structures, coupled to-the ratchet-like motion of the two subunits observed previously, suggests a mechanism for the final movements of messenger RNA (mRNA) and transfer RNAs (tRNAs) during translocation.