The crucial role of mechanical heterogeneity in regulating follicle development and ovulation with engineered ovarian microtissue.
The crucial role of mechanical heterogeneity in regulating follicle development and ovulation with engineered ovarian microtissue.
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DOI：
10.1016/j.biomaterials.2014.03.028
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发表时间：
2014-06
期刊：
影响因子：
14
通讯作者：
He, Xiaoming
中科院分区：
文献类型：
--
作者：
Choi, Jung Kyu;Agarvval, Pranay;Huang, Haishui;Zhao, Shuting;He, Xiaoming
目前的卵泡体外培养系统不能再现哺乳动物卵巢的机械异质性。在这里，我们报告的仿生卵巢微组织的微型化三维（3D）培养早期次级腔前卵泡的微流体生成，通过使用藻酸盐（硬）和胶原蛋白（软）制造卵巢皮质和髓质组织，分别。这种仿生的结构大大促进了卵泡发育到窦期。此外，它使体外排卵的卵丘卵母细胞复合体（COC）从窦卵泡在黄体生成素（LH）和表皮生长因子（EGF）的情况下，是公认的负责排卵在当代文献。这些数据揭示了哺乳动物卵巢中机械异质性在调节卵泡发育和排卵中的关键作用。本研究开发的仿生卵巢微组织和微流体技术对于改善卵泡体外培养以保持生育能力以及了解卵泡发育和排卵机制以促进寻找卵巢疾病导致不孕的治疗方法具有价值。
Contemporary systems for in vitro culture of ovarian follicles do not recapitulate the mechanical heterogeneity in mammalian ovary. Here we report microfluidic generation of biomimetic ovarian microtissue for miniaturized three-dimensional (3D) culture of early secondary preantral follicles by using alginate (harder) and collagen (softer) to fabricate the ovarian cortical and medullary tissues, respectively. This biomimetic configuration greatly facilitates follicle development to antral stage. Moreover, it enables in vitro ovulation of cumulus-oocyte complex (COC) from the antral follicles in the absence of luteinizing hormone (LH) and epidermal growth factor (EGF) that are well accepted to be responsible for ovulation in contemporary literature. These data reveal the crucial role of mechanical heterogeneity in the mammalian ovary in regulating follicle development and ovulation. The biomimetic ovarian microtissue and the microfluidic technology developed in this study are valuable for improving in vitro culture of follicles to preserve fertility and for understanding the mechanism of follicle development and ovulation to facilitate the search of cures to infertility due to ovarian disorders.
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影响因子：
3.7
作者：
Choi JK;He X
通讯作者：
He X
DOI：
10.1126/science.1171396
发表时间：
2009-05-15
期刊：
Science (New York, N.Y.)
影响因子：
--
作者：
Fan HY;Liu Z;Shimada M;Sterneck E;Johnson PF;Hedrick SM;Richards JS
通讯作者：
Richards JS
影响因子：
--
作者：
Shimada, Masayuki;Hernandez-Gonzalez, Inmaculada;Richards, JoAnne S.
通讯作者：
Richards, JoAnne S.
影响因子：
4.6
作者：
Cubaud, Thomas;Mason, Thomas G.
通讯作者：
Mason, Thomas G.
影响因子：
4.1
作者：
Choi, Jung Kyu;Agarwal, Pranay;He, Xiaoming
通讯作者：
He, Xiaoming