Comparative investigation of coarse and fine wool sheep skin indicates the early regulators for skin and wool diversity
Comparative investigation of coarse and fine wool sheep skin indicates the early regulators for skin and wool diversity
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DOI：
10.1016/j.gene.2020.144968
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发表时间：
2020-10-01
期刊：
影响因子：
3.5
通讯作者：
Mou, Chunyan
中科院分区：
文献类型：
--
作者：
Li, Shaomei;Chen, Wenshuo;Mou, Chunyan
毛囊是一个出色的微型系统，说明了器官发生和再生的机制。虽然在小鼠和鸡模型中广泛研究了调节皮肤和毛囊发育的一般机制，但调节皮肤和毛发多样性的微妙网络仍不清楚。绵羊是一个额外的模型，以解决在自然界中观察到的各种羊毛特性。对不同纤维类型的粗毛和细毛羊的初生毛囊大小和皮肤厚度进行了研究。通过RNA测序分析研究了两个绵羊品系在初级毛囊诱导阶段皮肤中的分子动力学，以产生1994个差异表达基因，揭示了与皮肤和毛囊形态差异高度相关的上皮细胞（6个基因）、真皮冷凝物（38个基因）和真皮成纤维细胞（58个基因）的标记基因。DEG在以上皮细胞迁移和分化、毛囊发育和外胚层基板形成的调节为代表的GO术语以及以WNT和Hedgehog信号通路为代表的控制皮肤结构差异的KEGG通路中富集。9个基因的qPCR检测证实了与RNA测序图谱相似的表达趋势。本研究通过对粗、细羊毛绵羊皮肤的比较研究，揭示了初级毛囊诱导阶段皮肤和毛囊的差异，并指出了在初级毛囊的早期形态发生过程中，潜在的效应因子（APCDD 1、FGF 20、DKK 1、IGFBP3和SFRP 4）调节着皮肤区室，从而在后期发育阶段形成可变的羊毛纤维厚度。
The hair follicle is an excellent mini-system illustrating the mechanisms governing organogenesis and regeneration. Although the general mechanisms modulating skin and hair follicle development are widely studied in mouse and chicken models, the delicate network regulating skin and hair diversity remains largely unclear. Sheep is an additional model to address the various wool characteristics observed in nature. The coarse and fine wool sheep with diverse fibers were examined to show differences in the primary wool follicle size and skin thickness. The molecular dynamics in skin staged at the primary wool follicle induction between two sheep lines were investigated by RNA-sequencing analyses to generate 1994 differentially expressed genes revealing marker genes for epithelium (6 genes), dermal condensate (38 genes) and dermal fibroblast (58 genes) highly correlated with skin and wool follicle morphological differences. The DEGs were enriched in GO terms represented by epithelial cell migration and differentiation, regulation of hair follicle development and ectodermal placode formation, and KEGG pathways typified by WNT and Hedgehog signaling pathways governing the differences of skin structure. The qPCR detection of 9 genes confirmed the similar expression tendency with RNA-sequencing profiles. This comparative study of coarse and fine wool sheep skin reveals the presence of skin and wool follicle differences at primary wool follicle induction stage, and indicates the potential effectors (APCDD1, FGF20, DKK1, IGFBP3 and SFRP4) regulating the skin compartments during the early morphogenesis of primary wool follicles to shape the variable wool fiber thickness in later developmental stages.