White with partially pink flower color in Lilium cernuum var. album is caused by transcriptional regulation of anthocyanin biosynthesis genes
White with partially pink flower color in Lilium cernuum var. album is caused by transcriptional regulation of anthocyanin biosynthesis genes
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DOI：
10.1016/j.scienta.2019.108880
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发表时间：
2020-01
影响因子：
4.3
通讯作者：
M. Yamagishi
中科院分区：
文献类型：
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作者：
M. Yamagishi
百合是亚洲百合育种的重要野生种之一。L.cernuum包括两种花色类型：在所选的普通类型中，整个花被片为粉红色，而变种.albumflowers发育白色花被片，部分为粉红色。为了了解产生部分粉红色的白色花被片的机制，研究了与花青素苷生物合成相关的基因的表达。虽然白变种花被片的花色素苷含量明显低于普通型花被片，但它们都表达相同的LcMYB 12转录因子基因。我们发现该基因在使用烟草叶片的瞬时测定（农杆菌渗入）中正向调节花色素苷生物合成基因的表达。白变种中LcMYB 12和花青素合成基因的表达量低于普通型。因此，LcMYB 12表达的下调可能是产生部分着色的白色花被片的主要原因。白变种还具有LcMYB 12的一个非功能等位基因LcMYB 12-del，其C端区域被截短。然而，对普通型百合与白百合杂交F1代植株进行的分离分析表明，LcMYB 12-del表达与花被片颜色无关。除了LcMYB 12外，LcMYBSPLATTER也在花被片区域表达。由于LcMYBSPLATTER序列与LhMYBSPLATTER（以前的LhMYB 12-Latvia）的序列非常相似，调节花色素斑点的色素沉着，亚洲杂种百合中的LhMYBSPLATTER很可能来源于mL。cernuum。
Lilium cernuumis among the most important wild species used for the breeding of Asiatic hybrid lilies. Two flower-color types are included inL.cernuum: the entire tepals are pink in the selected common type, whereas var.albumflowers develop white tepals with partially pink regions. To understand the mechanisms creating the white with partially pink tepals, expression of genes related to anthocyanin biosynthesis was investigated. Although the amounts of anthocyanin pigments were considerably lower in the tepals of var.albumthan in those of the common type, they both expressed the identicalLcMYB12transcription factor gene. We found this gene to positively regulate the expression of anthocyanin biosynthesis genes in the transient assay (agroinfiltration) using tobacco leaves. Expression levels ofLcMYB12and anthocyanin biosynthesis genes were lower in var.albumthan in the common type. Thus, down-regulation ofLcMYB12expression is likely to be the main reason for the creation of the white with partially colored tepals. The var.albumalso possessed a non-functional allele ofLcMYB12,LcMYB12-del, in which the C-terminal region was truncated. However, the segregation analysis conducted using F1plants derived from the crosses between the common type and var.albumindicated thatLcMYB12-delexpression was not correlated with tepal colors.Lilium cernuumdeveloped tiny spots at the base of its tepals. In addition toLcMYB12,LcMYBSPLATTERwas expressed at the spot-containing tepal region. Because theLcMYBSPLATTERsequence was very similar to the sequence ofLhMYBSPLATTER(formerlyLhMYB12-Latvia) regulating the pigmentation at splatter-type anthocyanin spots,LhMYBSPLATTERin Asiatic hybrid lilies is likely to have been derived fromL. cernuum.