Estrus Synchronization with Pseudopregnant Gilts Induced by a Single Treatment of Estradiol Dipropionate
Estrus Synchronization with Pseudopregnant Gilts Induced by a Single Treatment of Estradiol Dipropionate
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DOI：
10.1262/jrd.10-006t
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发表时间：
2010-08-01
影响因子：
1.8
通讯作者：
Wada, Yasunori
中科院分区：
文献类型：
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作者：
Noguchi, Michiko;Yoshioka, Koji;Wada, Yasunori
本研究旨在探讨二丙酸雌二醇(EDP)单独处理是否能诱导假孕母猪发情，以及PGF(2α)对EDP诱导假孕母猪同期发情的影响。在试验1中，母猪在第12天(第0天为发情开始)分别饲喂20 mg EDP(n=11)或赋形剂(n=5)。假孕的建立被定义为缺乏发情，并在12至36天期间将血浆孕酮浓度维持在1 ng/ml以上。经EDP处理的11头母猪中有9头(82%)假孕。直到29日龄，EDP处理组母猪的血浆雌二醇-17β水平显著高于对照组。在试验2中，从假孕母猪(n=6)或周期母猪(n=5)的第36天(n=6)或第10天(对照；n=5)开始，每隔24小时间隔两次注射PGF(2α)。83%的假孕母猪在PGF(2α)处理后发情。假孕母猪从第一次PGE(2α)处理至发情开始的时间和黄体生成素峰值的时间显著短于对照组。试验三，用前列腺素F(2α)处理后的发情期人工授精饲养6头假孕母猪。用PGE(2α)处理的假孕母猪和周期母猪的产仔率和平均产仔数没有差异。这些结果表明，在发情周期的第12天一次性处理EDP可以诱导猪的假孕，给EDP诱导的假孕猪注射PGF(2α)是一种简便的方案，可用于周期猪的发情同步程序。
The aims of this study were to determine whether a single treatment of estradiol dipropionate (EDP) could induce pseudopregnancy in gilts and to determine the effectiveness of PGF(2 alpha) treatment on estrus synchronization in EDP-induced pseudopregnant gilts. In experiment 1, gilts were treated with 20 mg of EDP (n=11) or vehicle (n=5) on Day 12 (Day 0=onset of estrus). Establishment of pseudopregnancy was defined as a lack of estrus and maintenance of the plasma progesterone concentration above 1 ng/ml between Days 12 and 36. Nine of 11 gilts (82%) treated with EDP became pseudopregnant. The plasma estradiol-17 beta level was significantly higher in the EDP-treated gilts than in the control gilts until Day 29. In experiment 2, PGF(2 alpha) was administered twice with a 24-h interval from Day 36 in pseudopregnant gilts (n=6) or Day 10 in cyclic gilts (control; n=5). Estrus after PGF(2 alpha) treatment was observed in 83% of the pseudopregnant gilts. The interval from the day of the first PGE(2 alpha) treatment to the onset of estrus and the peak of the LH surge was significantly shorter in the pseudopregnant gilts than in the control gilts. In experiment 3, six pseudopregnant gilts were bred by artificial insemination at the estrus after PGF(2 alpha) treatment. The farrowing rate and average litter size did not differ between the PGE(2 alpha)-treated pseudopregnant and cyclic gilts. These results indicate that a single treatment of EDP on Day 12 of the estrous cycle can induce pseudopregnancy in pigs and that a convenient protocol for administering PGF(2 alpha) to EDP-induced pseudopregnant pigs is available for estrus synchronization programs in cyclic pigs.