The pepper receptor-like cytoplasmic protein kinase CaPIK1 is involved in plant signaling of defense and cell-death responses
The pepper receptor-like cytoplasmic protein kinase CaPIK1 is involved in plant signaling of defense and cell-death responses
复制标题
DOI：
10.1111/j.1365-313x.2011.04525.x
复制
发表时间：
2011-05-01
期刊：
影响因子：
7.2
通讯作者：
Hwang, Byung Kook
中科院分区：
文献类型：
--
作者：
Kim, Dae Sung;Hwang, Byung Kook
某些蛋白激酶已被证明是至关重要的植物细胞信号通路与植物免疫反应。在这里，我们确定了辣椒（辣椒）受体样细胞质蛋白激酶（RLCK）基因（CaPIK 1），是转录激活的感染野油菜黄单胞菌pv。vesicatoria（Xcv）.辣椒植物中CaPIK 1的沉默赋予对Xcv感染的增强的易感性。水杨酸依赖的防御反应减弱的CaPIK 1沉默的植物，包括水杨酸依赖的基因的表达，但不是茉莉酸调控的基因。在CaPIK 1沉默的植物中，Xcv感染诱导水杨酸积累受到损害。功能性CaPIK 1蛋白不仅自身磷酸化，而且磷酸化髓鞘碱性蛋白。CaPIK 1存在于细胞质中，也通过其N-末端定位于植物细胞的质膜。CaPIK 1在辣椒叶片中的瞬时表达导致活性氧（ROS）的产生，最终导致过敏性细胞死亡。拟南芥中CaPIK 1的过表达（OX）增强了对假单胞菌pv.番茄和阿拉伯透明霜霉（Hyaloperonospora arabidopsidis），与升高的ROS爆发相关。CaPIK 1-OX植物中的水杨酸水平高于野生型植物中的水平。总之，这些结果表明，CaPIK 1调节水杨酸依赖性防御反应对病原体感染所需的信号传导。
P>Certain protein kinases have been shown to be crucial for plant cell signaling pathways associated with plant immune responses. Here we identified a pepper (Capsicum annuum) receptor-like cytoplasmic protein kinase (RLCK) gene (CaPIK1) that is transcriptionally activated by infection with Xanthomonas campestris pv. vesicatoria (Xcv). Silencing of CaPIK1 in pepper plants confers enhanced susceptibility to Xcv infection. Salicylic acid-dependent defense responses are attenuated in the CaPIK1-silenced plants, including expression of salicylic acid-dependent genes, but not of a jasmonic acid-regulated gene. Induction of salicylic acid accumulation by Xcv infection is compromised in CaPIK1-silenced plants. The functional CaPIK1 protein not only autophosphorylates, but also phosphorylates myelin basic protein. CaPIK1 exists in the cytoplasm and also localizes to the plasma membrane of plant cells via its N-terminus. Transient expression of CaPIK1 in pepper leaves leads to generation of reactive oxygen species (ROS), ultimately leading to hypersensitive cell death. Over-expression (OX) of CaPIK1 in Arabidopsis enhances the basal resistance to infection with Pseudomonas syringae pv. tomato and Hyaloperonospora arabidopsidis, associated with elevated ROS bursts. Salicylic acid levels in CaPIK1-OX plants are higher than those in wild-type plants. Together, these results suggest that CaPIK1 modulates the signaling required for the salicylic acid-dependent defense response to pathogen infection.