Suppressed DNA base pair stacking assembly of gold nanoparticles in an alcoholic solvent for enhanced ochratoxin A detection in Baijiu
Suppressed DNA base pair stacking assembly of gold nanoparticles in an alcoholic solvent for enhanced ochratoxin A detection in Baijiu
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在酒精溶剂中抑制金纳米粒子的 DNA 碱基对堆积组装，用于增强白酒中赭曲霉毒素 A 的检测
DOI：
10.1039/d3an00016h
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发表时间：
2023
期刊：
影响因子：
--
通讯作者：
Maeda Mizuo
中科院分区：
文献类型：
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作者：
He Zhiyu;Chen Qianyuan;Ding Shansen;Wang Guoqing;Takarada Tohru;Maeda Mizuo
目前建立的用于检测饮料中真菌毒素的DNA纳米探针受到复杂样品预处理和复杂体系中不可控纳米颗粒絮凝的限制。通过DNA功能化纳米金（DNA-AuNPs）的靶向调控碱基对堆积组装，建立了一种快速检测白酒中赭曲霉毒素A（OTA）的进样/“是”或“否”应答比色法。OTA的比色意义依赖于OTA与AuNP表面接枝的DNA在与OTA靶向适体结合时的竞争。适体对OTA的特异性识别可以防止AuNP表面形成DNA双链体，从而抑制DNA-AuNP的碱基对堆叠组装并产生“开启”颜色。通过使用凸出环设计和醇溶液进一步抑制DNA杂交，DNA-AuNP表现出对于OTA感测的改善的再现性，同时保持对OTA的优异的介电性。检测限为88 nM，沿着对OTA具有高度特异性，低于世界各国规定的食品中OTA的最大耐受水平。整个反应时间不到17分钟，避免了样品预处理。DNA-AuNPs具有抗干扰特性和灵敏的“开启”性能，可方便地现场检测日常饮料中的霉菌毒素。
The currently established DNA nanoprobes for the detection of mycotoxin from beverages have been limited by complicated sample pretreatment and uncontrollable nanoparticle flocculation in complex systems. We develop a rapid colorimetric approach for ochratoxin A (OTA) detection in Baijiu in a sample-in/“yes” or “no” answer-out fashion through target-modulated base pair stacking assembly of DNA-functionalized gold nanoparticles (DNA–AuNPs). The colorimetric signification of OTA relies on the competition of OTA with the AuNP surface-grafted DNA in binding with an OTA-targeted aptamer. The specific recognition of OTA by the aptamer prevents DNA duplex formation on the AuNP surface, thereby inhibiting the base pair stacking assembly of the DNA–AuNPs and giving rise to a “turn-on” color. By further suppressing DNA hybridization using a bulged loop design and an alcohol solution, the DNA–AuNPs exhibit an improved reproducibility for OTA sensing while maintaining excellent susceptivity to OTA. A detection limit of 88 nM was achieved along with high specificity towards OTA, which is lower than the maximum tolerated level of OTA in foodstuffs defined by countries worldwide. The entire reaction time, avoiding sample pretreatment, is less than 17 min. The DNA–AuNPs with anti-interference features and sensitive “turn-on” performance promise convenient on-site detection of mycotoxin from daily beverages.