Quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels onto positively charged nylon membranes.
Quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels onto positively charged nylon membranes.
复制标题
将十二烷基硫酸钠-聚丙烯酰胺凝胶中的蛋白质定量电转移到带正电的尼龙膜上。
DOI：
10.1016/0003-2697(87)90409-x
复制
发表时间：
1987
影响因子：
2.9
通讯作者：
Rosenberg,GH
中科院分区：
文献类型：
--
作者：
Peluso,RW;Rosenberg,GH
介绍了一种将蛋白质从十二烷基硫酸钠-聚丙烯酰胺凝胶中重复性和定量电转移到Zetabind或Gene Screen Plus膜上的方法。该程序使用市售的设备，包括三个关键参数：省略甲醇从转移缓冲液，使用薄（0.75毫米）解析凝胶，和一个新开发的协议预处理的聚丙烯酰胺凝胶电泳后和电印迹前。这些参数的组合产生了定性和定量反映原始聚丙烯酰胺凝胶中蛋白质的印迹。
A method for the reproducible and quantitative electrotransfer of proteins from sodium dodecyl sulfate-polyacrylamide gels to a single sheet of either Zetabind or Gene Screen Plus membranes is presented. This procedure uses commercially available equipment and includes three crucial parameters: the omission of methanol from the transfer buffer, the use of thin (0.75-mm) resolving gels, and a newly developed protocol for pretreatment of the polyacrylamide gel after electrophoresis and before electroblotting. This combination of parameters yields a blot that both qualitatively and quantitatively reflects the proteins in the original polyacrylamide gel.