PGP9.5, A UBIQUITIN C-TERMINAL HYDROLASE - PATTERN OF MESSENGER-RNA AND PROTEIN EXPRESSION DURING NEURAL DEVELOPMENT IN THE MOUSE
PGP9.5, A UBIQUITIN C-TERMINAL HYDROLASE - PATTERN OF MESSENGER-RNA AND PROTEIN EXPRESSION DURING NEURAL DEVELOPMENT IN THE MOUSE
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DOI：
10.1016/0165-3806(94)00217-n
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发表时间：
1995-04-18
期刊：
影响因子：
--
通讯作者：
EDWARDS, YH
中科院分区：
文献类型：
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作者：
SCHOFIELD, JN;DAY, INM;EDWARDS, YH
PGP 9.5是一种神经元和神经内分泌特异性泛素羧基末端水解酶，估计占总脑蛋白的1-2%。我们检查了发育中的小鼠胚胎中PGP9.5 mRNA和蛋白质的时间和空间分布。这些研究表明，PGP9.5在整个中枢和外周神经系统的所有发育阶段的所有分化神经元中都以高水平存在。在蛋白质之前1天和神经元分化进行之前，在神经管中检测到mRNA。mRNA和蛋白质都存在于运动和感觉神经元的最初出现期间，在它们的峰值产生之前。采用微波预处理切片检测PGP9.5免疫反应性，以揭示表位。一般而言，解蔽导致免疫反应性的整体增强，尽管在某些部位，例如背根和颅神经节和希氏束，有证据表明解蔽蛋白与解蔽蛋白的分布存在解剖学差异。PGP9.5的非常早期的表达表明，在神经元前体的分化以及在分化的神经元中存在泛素水解酶的作用。
PGP9.5 is a neurone and neuro-endocrine specific ubiquitin carboxyl terminal hydrolase estimated to form 1-2% of total brain protein. We have examined the temporal and spatial distribution of PGP9.5 mRNA and protein in the developing mouse embryo. These studies show that PGP9.5 is present at high levels in all differentiated neurones throughout the central and peripheral nervous systems at all stages of development. The mRNA is detected in the neural tube 1 day prior to the protein and before neuronal differentiation is underway. Both mRNA and protein are present during the initial appearance of motor and sensory neurones, prior to their peak production. PGP9.5 immunoreactivity was detected using microwave pretreatment of sections in order to unmask epitopes. In general unmasking led to an overall enhancement of immunoreactivity although in some sites, for example the dorsal root and cranial nerve ganglia and the bundle of His, there was evidence for anatomical variation in the distribution of masked versus unmasked protein. The very early expression of PGP9.5 suggests that there is a role for ubiquitin hydrolases in the differentiation of neurone precursors as well as in the differentiated neurone.