SELECTIVE DISRUPTION OF SARCOTUBULAR SYSTEM IN FROG SARTORIUS MUSCLE - A QUANTITATIVE STUDY WITH EXOGENOUS PEROXIDASE AS A MARKER
SELECTIVE DISRUPTION OF SARCOTUBULAR SYSTEM IN FROG SARTORIUS MUSCLE - A QUANTITATIVE STUDY WITH EXOGENOUS PEROXIDASE AS A MARKER
复制标题
DOI：
10.1083/jcb.39.2.451
复制
发表时间：
1968-01-01
影响因子：
7.8
通讯作者：
EISENBERG, RS
中科院分区：
文献类型：
--
作者：
EISENBERG, B;EISENBERG, RS
在甘油-林格氏溶液中浸泡1小时，然后恢复正常林格氏溶液的骨骼肌具有破坏的肌管系统。这种效应与恢复到林格氏有关，因为肌肉在甘油-林格氏状态时具有正常的精细结构。Karnovsky的过氧化物酶法被发现是一个非常可靠的细胞外空间的标记，填充98.5%的正常肌肉中的小管。有趣的是，正常肌肉中只有84.1%的肌节具有横小管。甘油处理的肌纤维中基本上不存在肌管系统，仅2%的管状系统保持与细胞外间隙连接;完整的残余物是仅延伸到纤维中几个微米的残端。因此，甘油处理的肌纤维提供了几乎没有肌管系统的骨骼肌的制备。由于肌浆网没有被破坏，肌膜和肌丝在这种制备中是完整的，因此肌膜的性质可以与管状系统的性质分开。
Skeletal muscles which have been soaked for 1 hr in a glycerol-Ringer solution and then returned to normal Ringer solution have a disrupted sarcotubular system. The effect is associated with the return to Ringer's since muscles have normal fine structure while still in glycerol-Ringer's. Karnovsky's peroxidase method was found to be a very reliable marker of extracellular space, filling 98.5% of the tubules in normal muscle. It was interesting to note that only 84.1% of the sarcomeres in normal muscle have transverse tubules. The sarcotubular system was essentially absent from glycerol-treated muscle fibers, only 2 % of the tubular system remaining connected to the extracellular space; the intact remnants were stumps extending only a few micra into the fiber. Thus, glycerol-treated muscle fibers provide a preparation of skeletal muscle with little sarcotubular system. Since the sarcoplasmic reticulum is not destroyed and the sarcolemma and myofilaments are intact in this preparation, of the properties of the sarcolemma may thus be separated from those of the tubular system.