A SINE family widely distributed in the plant kingdom and its evolutionary history
A SINE family widely distributed in the plant kingdom and its evolutionary history
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DOI：
10.1007/s11103-006-0026-7
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发表时间：
2006-06-01
影响因子：
5.1
通讯作者：
Yasui, Yasuo
中科院分区：
文献类型：
--
作者：
Fawcett, Jeffrey A.;Kawahara, Taihachi;Yasui, Yasuo
研究了 Au SINE 在植物中的分布和演变。 Au SINE 是一种短散布元素，首次在小麦的近亲 Aegilops umbellulata 中发现。 Au SINE 以前曾在小麦、玉米、烟草和番茄等物种中发现，但在水稻中没有发现。在这项研究中，我们首先搜索了公共数据库，然后使用 Au 的内部引物通过 PCR 检测了多种植物物种中 Au 的存在。尽管包括水稻在内的许多物种中可能不含有金，但在许多禾本科、茄科和豆科物种以及基生被子植物物种三叶草中都发现了金。系统发育研究表明，Au SINE 起源于单子叶植物和真双子叶植物分化之前。对 Asimina、Triticum、Zea、Nicotiana、Lotus、Medicago 和 Glycine 的 Au SINE 序列进行了比对和比较。尽管Au序列在远缘物种中高度保守，但大豆中的每个Au元件都有16 bp的缺失，并且其3'端与其他物种的序列不同。这种类型的金仅存在于 G. max 中，而不存在于其他物种中，包括其他豆科物种，如 M. truncatula 和 L. japonicus。这是首次报道植物 SINE 家族存在于多个谱系中，并讨论了 Au SINE 在植物界，特别是禾本科和豆科中的进化。
The distribution and evolution of Au SINE in plants were examined. Au SINE is a short interspersed element first identified in Aegilops umbellulata, a close relative of wheat. The Au SINE was previously found in species such as wheat, maize, tobacco, and tomato, but not in rice. In this study, we first searched public databases, and next examined the presence of Au in a broad range of plant species by PCR using internal primers of Au. Although Au is likely to be absent from many species including rice, it was identified in many Gramineae, Solanaceae, and Fabaceae species, and also in a basal angiosperm species, Asimina triloba. Phylogenetic studies suggest that Au SINE originated before the divergence of monocots and eudicots. Au SINE sequences of Asimina, Triticum, Zea, Nicotiana, Lotus, Medicago, and Glycine were aligned and compared. Although sequences of Au were highly conserved among distantly related species, every Au element in Glycine had a 16 bp deletion and its 3' end differed from sequences of other species. This type of Au could only be found in G. max, and not in other species including other Fabaceae species such as M. truncatula and L. japonicus. This is the first report of a plant SINE family present in multiple lineages, and the evolution of Au SINE in the plant kingdom, especially in Gramineae and Fabaceae is discussed.