Escherichia coli binding to and invasion of brain microvascular endothelial cells derived from humans and rats of different ages
Escherichia coli binding to and invasion of brain microvascular endothelial cells derived from humans and rats of different ages
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DOI：
10.1128/iai.67.10.5522-5525.1999
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发表时间：
1999-10-01
影响因子：
3.1
通讯作者：
Kim, KS
中科院分区：
文献类型：
--
作者：
Stins, MF;Nemani, PV;Kim, KS
大肠杆菌脑膜炎通常发生在新生儿期，但这种年龄依赖性的基础尚不清楚。我们以前已经确定了两种类型的E。大肠杆菌脑微血管内皮细胞（BR IEC）的相互作用有助于E。大肠杆菌穿过血脑屏障（即，结合和入侵）。本研究探讨了年龄依赖是否关闭。所谓脑膜炎是由于新生儿和成人BMEC与大肠杆菌相互作用的能力不同所致。电话从不同年龄的大鼠（10天、20天和3个月）以及不同年龄的人（胎儿、4- 7岁儿童、35岁成人和60- 85岁老年人）中分离BMEC。把捆绑解开。E.对于来自年轻和年老大鼠以及来自人胎儿、儿童、成人和老年人的BMEC，侵入BMEC的呼唤是相似的。这些发现表明E.新生儿中的大肠杆菌脑膜炎不太可能是由于新生儿与成人BMEC相比具有更大的结合和侵袭能力。
Escherichia coli meningitis commonly occurs in the neonatal period, but the basis of this age dependency is unclear. We have previously identified two types of E. coli-brain microvascular endothelial cell (BR IEC) interactions contributing to E. coli traversal of the blood-brain barrier (i.e., binding and invasion). The present study examined whether the age dependency off. call meningitis stemmed from differences in the capacities of neonatal and adult BMECs to interact dth E. call. BMECs were isolated from rats of different ages (10 days, 20 days and 3 months) as well as from humans of different ages (fetuses, 4- to 7-year-old children, and a 35-year-old adult, and 60- to 85-year-old geriatrics). The bindings off. call to young and old rat BMECs were similar, Also, the abilities of E. call to invade BMECs were similar for BMECs derived from young and old rats and from human fetuses, children, adults, and geriatrics. These findings suggest that the predominance of E. coli meningitis in neonates is not likely due to greater binding and invasion capacities of newborn compared to adult BMECs.