High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis
High‐frequency pulsed diode laser irradiation inhibits bone resorption in mice with ligature‐induced periodontitis
复制标题
高频脉冲二极管激光照射抑制结扎引起的牙周炎小鼠的骨吸收
DOI：
10.1111/jcpe.13695
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发表时间：
2022
影响因子：
6.7
通讯作者：
Aoki Akira
中科院分区：
文献类型：
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作者：
Ohsugi Yujin;Hatasa Masahiro;Katagiri Sayaka;Hirota Tomomitsu;Shimohira Tsuyoshi;Shiba Takahiko;Komatsu Keiji;Tsuchiya Yosuke;Fukuba Shunsuke;Lin Peiya;Toyoshima Keita;Maekawa Shogo;Niimi Hiromi;Iwata Takanori;Aoki Akira
目的探讨高频脉冲二极管激光对结扎性牙周炎小鼠骨吸收的抑制作用及其对牙龈组织基因表达和微生物组成的生物学效应。材料与方法对小鼠牙龈组织进行牙周结扎和（或）激光照射：Co（不结扎、不激光照射）、Li（不结扎、不激光照射）、La（不结扎、有激光照射）、LiLa（有激光照射结扎）。采用微计算机断层扫描评估骨吸收。结扎后3天对四组患者的牙龈组织进行RNA - seq分析。根据16S rRNA基因序列的数量来评估Li和LiLa之间微生物组成的差异。结果激光照射可明显抑制结扎术后骨吸收。在Co和La牙龈组织中，RNA‐seq显示了许多差异表达的基因，提示二极管激光照射改变了基因表达。基因集富集分析显示，与Co和Li相比，La和LiLa的牙龈组织中mTORC1信号和E2F靶基因集分别富集。激光照射减少了结扎物中提取的DNA量，改变了Li和LiLa之间的细菌网络结构。结论高频脉冲二极管激光治疗结扎性牙周炎具有明显的生物效应和抑制骨吸收的作用。
AimThe purpose of this study was to elucidate the suppressive effect of high‐frequency pulsed diode laser irradiation on bone resorption and its biological effects on gene expression and microbiome composition on the gingival tissue in ligature‐induced periodontitis in mice.Materials and MethodsLigating ligature around the teeth and/or laser irradiation was performed on the gingival tissue in mice as follows: Co (no ligature and no laser irradiation), Li (ligation without laser irradiation), La (no ligature but with laser irradiation), and LiLa (ligation with laser irradiation). Bone resorption was evaluated using micro‐computed tomography. RNA‐seq analysis was performed on gingival tissues of all four groups at 3 days after ligation. The differences in microbial composition between Li and LiLa were evaluated based on the number of 16S rRNA gene sequences.ResultsBone resorption caused by ligation was significantly suppressed by laser irradiation. RNA‐seq in Co and La gingival tissue revealed many differentially expressed genes, suggesting diode laser irradiation altered gene expression. Gene set enrichment analysis revealed mTORC1 signalling and E2F target gene sets were enriched in gingival tissues both in La and LiLa compared with that in Co and Li, respectively. The amount of extracted DNA from ligatures was reduced by laser irradiation, and bacterial network structure was altered between the Li and LiLa.ConclusionsHigh‐frequency pulsed diode laser irradiation showed biological effects and suppressed bone resorption in ligature‐induced periodontitis.